Rat, Cow, Dog, Zebrafish
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Nkx2.2.
This antibody gave a positive signal in Human placenta tissue lysate as well as the following whole cell lysates: U373; U87mg.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 30 kDa).
May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain.
The homeodomain is essential for interaction with OLIG2.
Information by UniProt
Homeobox protein NK 2 homolog B antibody
Homeobox protein NK-2 homolog B antibody
Homeobox protein Nkx 2.2 antibody
Western blot - Nkx2.2 antibody (ab79916)
All lanes :
Anti-Nkx2.2 antibody (ab79916) at 1 µg/ml
Lane 1 :
Human placenta tissue lysate - total protein (
Lane 2 :
U373 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate (
Lane 3 :
U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
38 kDa (
why is the actual band size different from the predicted?
Additional bands at:
27 kDa, 70 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
The predicted molecular weight of Nkx2.2 is 30 kDa (SwissProt), however we expect to observe a banding pattern at 38 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This product has been referenced in:
et al. C-Abl inhibitor imatinib enhances insulin production by ß cells: c-Abl negatively regulates insulin production via interfering with the expression of NKx2.2 and GLUT-2. PLoS One 9:e97694 (2014).
Read more (PubMed: 24835010) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"