产品名称 Anti-Nkx2.2抗体 参阅全部 Nkx2.2 一抗
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Rat, Cow, Dog, Zebrafish
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Nkx2.2.
This antibody gave a positive signal in Human placenta tissue lysate as well as the following whole cell lysates: U373; U87mg.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 30 kDa).
功能 May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
序列相似性 Belongs to the NK-2 homeobox family. Contains 1 homeobox DNA-binding domain.
结构域 The homeodomain is essential for interaction with OLIG2.
Information by UniProt
Homeobox protein NK 2 homolog B antibody
Homeobox protein NK-2 homolog B antibody
Homeobox protein Nkx 2.2 antibody
Anti-Nkx2.2 antibody 图像
Western blot - Nkx2.2 antibody (ab79916)
All lanes : Anti-Nkx2.2 antibody (ab79916) at 1 µg/ml Lane 1 : Human placenta tissue lysate - total protein ( ab29745) Lane 2 : U373 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate ( ab14902) Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 30 kDa Observed band size : 38 kDa ( why is the actual band size different from the predicted?) Additional bands at : 27 kDa,70 kDa,78 kDa. We are unsure as to the identity of these extra bands. Exposure time : 3 minutes
Anti-Nkx2.2 antibody (ab79916)参考文献
This product has been referenced in:
et al. C-Abl inhibitor imatinib enhances insulin production by ß cells: c-Abl negatively regulates insulin production via interfering with the expression of NKx2.2 and GLUT-2. PLoS One 9:e97694 (2014).
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