概述

  • 产品名称Anti-Nkx1-2抗体
  • 描述
    兔多克隆抗体to Nkx1-2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 101-150 (AHQPERWQGV HEGSPEARAV AVGTEESGAE GLPASPGSPG SPRPRRRRAE) of Mouse Nkx1-2 (NP_033149).

  • 阳性对照
    • Mouse lung lysate

性能

应用

Our Abpromise guarantee covers the use of ab105940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 23457544

靶标

  • 功能May function in cell specification, particularly in the CNS.
  • 组织特异性Expression detected in adult brain, testis and spleen. In the testis, expressed in the germ cells of the seminiferous epithelium, in elongating spermatids and in spermatozoa. Expressed throughout the brain with highest levels in regions of the cerebral cortex, hippocampus, diencephalon, pons, medulla and cerebellum. In the embryo, expressed in the developing posterior central nervous system. First seen in the ectoderm lateral to the primitive streak, later it encompasses the neural plate. Starting at day 9.5 pc, expressed in distinct areas of spinal cord, hindbrain, midbrain and forebrain.
  • 序列相似性Belongs to the NK-1 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段In the embryo, expressed at highest levels at day 10 with levels decreasing during further development.
  • 翻译后修饰Phosphorylated by HIPK2 in vitro.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BB238F13.2 antibody
    • C10orf121 antibody
    • Homeobox protein SAX-1 antibody
    • NK1 homeobox 2 antibody
    • NK1 transcription factor related locus 2 antibody
    • NK1 transcription factor-related protein 2 antibody
    • NKX-1.1 antibody
    • NKX1-2 antibody
    • NKX12_MOUSE antibody
    • Sax1 antibody
    • Sax1-like antibody
    • Spinal cord axial homeobox gene 1 antibody
    see all

Anti-Nkx1-2 antibody 图像

  • Anti-Nkx1-2 antibody (ab105940) at 1 µg/ml + Mouse lung lysate at 10 µg

    Predicted band size : 32 kDa

Anti-Nkx1-2 antibody (ab105940)参考文献

This product has been referenced in:
  • Buniello A  et al. Headbobber: a combined morphogenetic and cochleosaccular mouse model to study 10qter deletions in human deafness. PLoS One 8:e56274 (2013). IHC-P ; Mouse . Read more (PubMed: 23457544) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse embryonic tissue, HF-stage embryo, E8.5 tail)
Permeabilization Yes - PBST (PBS / 0.1% v/v Triton X-100 (Sigma))
Specification Mouse embryonic tissue, HF-stage embryo, E8.5 tail
Blocking step 3% donkey serum / 1% BSA (Life Technologies) in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 21 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spinal Cord)
Specification Spinal Cord
Fixative Formaldehyde
Permeabilization Yes - Triton X-100 0.1%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Apr 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"