Anti-NKG2D抗体(ab203353)
Key features and details
- Rabbit polyclonal to NKG2D
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-NKG2D抗体
参阅全部 NKG2D 一抗 -
描述
兔多克隆抗体to NKG2D -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Rat NKG2D aa 100-200 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O70215 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab203353于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
1/20 - 1/100.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(or 1/50 -1/200 using a fluorescent secondary antibody). |
说明 |
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Flow Cyt
1/20 - 1/100. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (or 1/50 -1/200 using a fluorescent secondary antibody). |
靶标
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功能
Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes. -
组织特异性
Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs). -
序列相似性
Contains 1 C-type lectin domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 100528032 Human
- Entrez Gene: 22914 Human
- Entrez Gene: 27007 Mouse
- Omim: 611817 Human
- SwissProt: P26718 Human
- SwissProt: O54709 Mouse
- Unigene: 387787 Human
- Unigene: 8217 Mouse
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别名
- CD314 antibody
- CD314 antigen antibody
- D12S2489E antibody
see all
图片
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Immunohistochemical analysis of formalin-fixed, paraffin embedded Human nasopharyngeal carcinoma tissue labeling NKG2D with ab203353 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Mouse thymus cells probed with ab203353 (green) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
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ab203353 staining NKG2D in mouse lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 20 minutes at 37°C; antigen retrieval was by heat mediation in a citrate buffer for 15 minutes. Samples were incubated with primary antibody (1/400 in blocking buffer) overnight at 4°C, followed by a conjugated secondary and DAB staining.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab203353 被引用在 9 文献中.
- Wang H et al. CDX2 enhances natural killer cell-mediated immunotherapy against head and neck squamous cell carcinoma through up-regulating CXCL14. J Cell Mol Med 25:4596-4607 (2021). PubMed: 33733587
- Lee GH et al. Clinical Impact of Natural Killer Group 2D Receptor Expression and That of Its Ligand in Ovarian Carcinomas: A Retrospective Study. Yonsei Med J 62:288-297 (2021). PubMed: 33779082
- Čepcová D et al. The protective effect of 1-methyltryptophan isomers in renal ischemia-reperfusion injury is not exclusively dependent on indolamine 2,3-dioxygenase inhibition. Biomed Pharmacother 135:111180 (2021). PubMed: 33433354
- Spielmann J et al. Effects of obesity on NK cells in a mouse model of postmenopausal breast cancer. Sci Rep 10:20606 (2020). PubMed: 33244094
- Kravtsova-Ivantsiv Y et al. Excess of the NF-?B p50 subunit generated by the ubiquitin ligase KPC1 suppresses tumors via PD-L1- and chemokines-mediated mechanisms. Proc Natl Acad Sci U S A 117:29823-29831 (2020). PubMed: 33168738
- Mackiewicz Z et al. Sjögren's Syndrome: Concerted Triggering of Sicca Conditions. J Immunol Res 2019:2075803 (2019). PubMed: 30723748
- Zhang K et al. Splenic NKG2D confers resilience versus susceptibility in mice after chronic social defeat stress: beneficial effects of (R)-ketamine. Eur Arch Psychiatry Clin Neurosci N/A:N/A (2019). PubMed: 31875248
- Mo Z et al. Ultrasound-guided radiofrequency ablation enhances natural killer-mediated antitumor immunity against liver cancer. Oncol Lett 15:7014-7020 (2018). PubMed: 29725428
- Yin X et al. Role of NKG2D in cytokine-induced killer cells against lung cancer. Oncol Lett 13:3139-3143 (2017). PubMed: 28529563