兔多克隆抗体to NKF3 kinase family member
antigen sequence: DGQSICGELS IQEHCENKPV IIGWNRNRAA LSQKPLNNNN EDDEGISHVP KPYGNNDSAK KMSDNNNGLT EVLKEIAGLD TAPQIR, corresponding to amino acids 81-166 of Human NKF3 kinase family member
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro.
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with F-actin in serum-rich medium. Actin colocalization is reduced during serum starvation.
Information by UniProt
- FLJ21140 antibody
- FLJ34483 antibody
- KIAA2002 antibody
Immunocytochemistry/ Immunofluorescence - Anti-NKF3 kinase family member antibody (ab121865)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121865 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKF3 kinase family member antibody (ab121865)
ab121865, at a 1/10 dilution, staining NKF3 kinase family member in glandular cells of paraffin-embedded Human stomach tissue by immunohistochemistry.
has not yet been referenced specifically in any publications.
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