Anti-NKF3 kinase family member抗体(ab121865)

概述

  • 产品名称Anti-NKF3 kinase family member抗体
    参阅全部 NKF3 kinase family member 一抗
  • 描述
    兔多克隆抗体to NKF3 kinase family member
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: DGQSICGELS IQEHCENKPV IIGWNRNRAA LSQKPLNNNN EDDEGISHVP KPYGNNDSAK KMSDNNNGLT EVLKEIAGLD TAPQIR, corresponding to amino acids 81-166 of Human NKF3 kinase family member

  • 阳性对照
    • Human stomach tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab121865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 功能Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.
  • 序列相似性Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro.
  • 细胞定位Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with F-actin in serum-rich medium. Actin colocalization is reduced during serum starvation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ21140 antibody
    • FLJ34483 antibody
    • KIAA2002 antibody
    • NKF3 kinase family member antibody
    • PEAK1 antibody
    • PEAK1_HUMAN antibody
    • pseudopodium enriched atypical kinase antibody
    • Pseudopodium-enriched atypical kinase 1 antibody
    • SGK269 antibody
    • Sugen kinase 269 antibody
    • Tyrosine-protein kinase SgK269 antibody
    see all

Anti-NKF3 kinase family member antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121865 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121865, at a 1/10 dilution, staining NKF3 kinase family member in glandular cells of paraffin-embedded Human stomach tissue by immunohistochemistry.

Anti-NKF3 kinase family member antibody (ab121865)参考文献

ab121865 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab121865.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"