概述

  • 产品名称
  • 描述
    兔多克隆抗体to NKCC1
  • 特异性
    This antibody reacts specifically with human 132 kDa NKCC1 protein.
  • 经测试应用
    适用于: IHC-FoFr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from the N terminal region of human NKCC1.

性能

应用

Our Abpromise guarantee covers the use of ab59791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 21461058
WB 1/100 - 1/1000. Predicted molecular weight: 130 kDa.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能
    Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
  • 组织特异性
    Expressed in many tissues.
  • 序列相似性
    Belongs to the SLC12A transporter family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basolateral Na-K-Cl symporter antibody
    • BSC antibody
    • BSC2 antibody
    • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 antibody
    • mBSC2 antibody
    • MGC104233 antibody
    • NKCC1 antibody
    • PPP1R141 antibody
    • Protein phosphatase 1, regulatory subunit 141 antibody
    • S12A2_HUMAN antibody
    • Slc12a2 antibody
    • sodium-potassium-chloride cotransporter 1 antibody
    • Solute carrier family 12 (sodium/potassium/chloride transporter), member 2 antibody
    • solute carrier family 12 (sodium/potassium/chloride transporters) member 2 antibody
    • Solute carrier family 12 member 2 antibody
    • sy-ns antibody
    see all

Anti-NKCC1 antibody 图像

  • ab59791 staining NKCC1 in Mouse salivary gland tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/600 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab59791 staining NKCC1 in Rat brain stem tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Trition and blocked with 5% BSA for 3 hours at 20°C; antigen retrieval was by heat mediation in a citrate sodium buffer. Samples were incubated with primary antibody (1/1000) for 15 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • IHC image of ab59791 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59791, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-NKCC1 antibody (ab59791)参考文献

This product has been referenced in:
  • Arany S  et al. Nanoparticle-mediated Gene Silencing Confers Radioprotection to Salivary Glands In Vivo. Mol Ther 21:1182-94 (2013). Mouse . Read more (PubMed: 23511246) »
  • Murguía-Castillo J  et al. NKCC1 and KCC2 protein expression is sexually dimorphic in the hippocampus and entorhinal cortex of neonatal rats. Neurosci Lett 552:52-7 (2013). WB . Read more (PubMed: 23932891) »

See all 6 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Ferret Tissue sections (P0-Visual Cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1x Citrate Buffer
Permeabilization
Yes - 0.5% Triton in 1x PBS for 1 hour
Specification
P0-Visual Cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Francis Djankpa

Verified customer

提交于 Apr 29 2016

Application
Western blot
Sample
Ferret Tissue lysate - whole (parietal cortex in Brain)
Gel Running Conditions
Reduced Denaturing (3-8% Tris-acetate gel)
Loading amount
60 µg
Specification
parietal cortex in Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Francis Djankpa

Verified customer

提交于 Mar 03 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Dec 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Dec 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Salivary gland)
Specification
Salivary gland
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 03 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (Salivary glands)
Specification
Salivary glands
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. ABEER SHAALAN

Verified customer

提交于 Sep 26 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
Human Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 09 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
None
Sample
Guinea pig Tissue sections (hippocampus)
Specification
hippocampus
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 03 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Antigen retrieval step
None
Sample
Cat Tissue sections (hippocompus)
Specification
hippocompus
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 02 2013

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Chicken Tissue sections (hippocampus)
Specification
hippocampus
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 02 2013

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