Anti-NKAP抗体(ab121121)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to NKAP
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 20-132 of Human NKAP (Q8N5F7).

  • 阳性对照
    • Human testis tissue, A431 cells

性能

应用

Our Abpromise guarantee covers the use of ab121121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

靶标

  • 功能
    Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter.
  • 序列相似性
    Belongs to the UPF0396 family.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NF-kappa-B-activating protein antibody
    • NFKB activating protein antibody
    • Nkap antibody
    • NKAP_HUMAN antibody
    • Nuclear NF kappaB activating protein antibody
    see all

图片

  • ab121121 at 1/60 dilution staining NKAP in Paraffin-emebedded Human testis tissue by Immunohistochemistry. Note: strong nuclear positivity in seminiferus ducts.
  • Immunofluorescent staining of Human cell line A431 using ab121121 at a concentration of 4 µg/mg. Note: positivity in nucleus but not nucleoli.
  • All lanes : Anti-NKAP antibody (ab121121) at 1/500 dilution

    Lane 1 : HEK293 whole cell lysate
    Lane 2 : HELA whole cell lysate
    Lane 3 : HCT116 whole cell lysate
    Lane 4 : MEF whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

文献

ab121121 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10 % GEL, SDS)
Sample
Human Cell lysate - whole cell (HEK293, HELA, HCT116)
Specification
HEK293, HELA, HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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提交于 Apr 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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