Anti-NKAP抗体(ab121121)

概述

性能

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应用

Our Abpromise guarantee covers the use of ab121121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

靶标

  • 功能Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter.
  • 序列相似性Belongs to the UPF0396 family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NF-kappa-B-activating protein antibody
    • NFKB activating protein antibody
    • Nkap antibody
    • NKAP_HUMAN antibody
    • Nuclear NF kappaB activating protein antibody
    see all

Anti-NKAP antibody 图像

  • ab121121 at 1/60 dilution staining NKAP in Paraffin-emebedded Human testis tissue by Immunohistochemistry. Note: strong nuclear positivity in seminiferus ducts.
  • Immunofluorescent staining of Human cell line A431 using ab121121 at a concentration of 4 µg/mg. Note: positivity in nucleus but not nucleoli.
  • All lanes : Anti-NKAP antibody (ab121121) at 1/500 dilution

    Lane 1 : HEK293 whole cell lysate
    Lane 2 : HELA whole cell lysate
    Lane 3 : HCT116 whole cell lysate
    Lane 4 : MEF whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-NKAP antibody (ab121121)参考文献

ab121121 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10 % GEL, SDS)
Sample Human Cell lysate - whole cell (HEK293, HELA, HCT116)
Specification HEK293, HELA, HCT116
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Apr 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"