Store at Room Temperature. The product can be stored for up to 12 months.
Soluble in DMSO to 50 mM and in ethanol to 25 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ab2770 staining aryl hydrocarbon receptor in MDA-MB-231 cells treated with nitrendipine (ab120139), by ICC/IF. Increase in aryl hydrocarbon receptor expression correlates with increased concentration of nitrendipine, as described in literature. The cells were incubated at 37°C for 6h in media containing different concentrations of ab120139 (nitrendipine) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2770 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
McCarthy RT & Fry HK Nitrendipine block of calcium channel currents in vascular smooth muscle and adrenal glomerulosa cells. J Cardiovasc Pharmacol12 Suppl 4:S98-101 (1988).
Read more (PubMed: 2468885) »
Weiland GA & Oswald RE The mechanism of binding of dihydropyridine calcium channel blockers to rat brain membranes. J Biol Chem260:8456-64 (1985).
Read more (PubMed: 3159733) »
Stoepel K et al. Pharmacological studies of the antihypertensive effect of nitrendipine. Arzneimittelforschung31:2056-61 (1981).
Read more (PubMed: 7199297) »