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ab112149 is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential. The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
ab112149 uses our proprietary cationic NIR probe for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. In normal cells, the red fluorescence intensity is increased when the MitoNIR Dye is accumulated in the mitochondria. However, in apoptotic cells, the NIR stain intensity is decreased following the collapse of MMP. Cells stained with NIR Dye can be visualized with a flow cytometer at red excitation and far red emission (FL4 channel).
ab112149 provides all the essential components. ab112149 can be used together with other reagents, such as blue laser excited propidium iodide and for studying cell vitality and apoptosis. ab112149 is optimized for screening apoptosis activators and inhibitors with a flow cytometer.
Visit our FAQs page for tips and troubleshooting.
ab112149 should be stored dessicated.
|Assay Buffer||1 x 100ml|
|MitoNIR Dye||1 x 500µl|
Our Abpromise guarantee covers the use of ab112149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
Fig. The decrease in fluorescence intensity of MitoNIR Dye with the addition of CCCP (ab141229) in Jurkat cells.
Cells were loaded with MitoNIR Dye1x in the presence of DMSO alone (red),1µM CCCP(orange), 10µM CCCP(blue) and 50µM CCCP(green).
Fig. The decrease in fluorescence intensity of MitoNIR Dye with the addition of CCCP (ab141229) in HL60 cells.
Cells were loaded with MitoNIR Dye1x in the presence of DMSO alone (red), 1µM CCCP (blue), 10µM CCCP (orange) and 50µM CCCP (green).
ab112149 has not yet been referenced specifically in any publications.