概述

  • 产品名称Anti-NIPA抗体
    参阅全部 NIPA 一抗
  • 描述
    兔多克隆抗体to NIPA
  • 特异性detects endogenous levels of total NIPA protein.
  • 经测试应用适用于: ELISA, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    synthesized non-phosphopeptide derived from human NIPA around the phosphorylation site of serine 354 (T-R-SP-W-D).

  • 阳性对照
    • HeLa cells, extract from COS7 cells treated with HU (2nM, 24hours) IHC-P: FFPE human testis tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab63375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF 1/500 - 1/1000.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.
  • 组织特异性Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 C3HC-type zinc finger.
  • 发展阶段Weakly expressed in G0/G1 phases, abundant during S and G2/M phases, and strongly decreases thereafter.
  • 结构域The F-box-like region is required for the interaction with SKP1.
  • 翻译后修饰Phosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-354 phosphorylation, a major site during the course of cell-cycle-dedendent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hNIPA antibody
    • NIPA_HUMAN antibody
    • Nuclear interacting partner of ALK antibody
    • Nuclear interacting partner of anaplastic lymphoma kinase antibody
    • Nuclear-interacting partner of ALK antibody
    • Nuclear-interacting partner of anaplastic lymphoma kinase antibody
    • zc3hc1 antibody
    • Zinc finger C3HC type containing 1 antibody
    • Zinc finger C3HC-type protein 1 antibody
    see all

Anti-NIPA antibody 图像

  • IHC image of NIPA staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63375, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunofluorescence analysis of HeLa cells, using 1/500 ab63375. Left: untreated sample. Right: sample treated with immunising peptide

Anti-NIPA antibody (ab63375)参考文献

ab63375 has not yet been referenced specifically in any publications.

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