概述

  • 产品名称Anti-NINL抗体
    参阅全部 NINL 一抗
  • 描述
    兔多克隆抗体to NINL
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 682-787 of Human NINL.

  • 阳性对照
    • Human cerebellum tissue.

性能

应用

Our Abpromise guarantee covers the use of ab121110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Involved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis.
  • 组织特异性Expressed in KYSE150 esophageal carcinoma, HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in adult brain, testis, kidney and retina.
  • 序列相似性Contains 4 EF-hand domains.
  • 结构域The KEN and D (destructive) boxes are required for the cell cycle-controlled NINL degradation by the APC/C pathway.
  • 翻译后修饰Phosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin.
    Ubiquitinated by the APC/C complex leading to its degradation.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome. Cytoplasm. In interphase cells, NINL is transported to the centrosomes by the dynein-dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4930519N13Rik antibody
    • dJ691N24.1 antibody
    • Gm1004, antibody
    • Gm1634 antibody
    • mKIAA0980 antibody
    • Ninein like antibody
    • Ninein-like protein antibody
    • NINL antibody
    • NINL_HUMAN antibody
    • Nlp antibody
    • RGD1306152 antibody
    • RP23-193L22.3 antibody
    • RP4-691N24.1 antibody
    see all

Anti-NINL antibody 图像

  • ab121101 at 1/100 dilution staining NINL in Paraffin-embedded Human Cerebellum tissue by Immunohistochemistry.
    Note: moderate cytoplasmic positivity in purkinje cells.

Anti-NINL antibody (ab121110)参考文献

This product has been referenced in:
  • Yin L  et al. Epidermal gene expression and ethnic pigmentation variations among individuals of Asian, European and African ancestry. Exp Dermatol 23:731-5 (2014). IHC-P ; Human . Read more (PubMed: 25055985) »

See 1 Publication for this product

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