Anti-Ninein抗体- Aminoterminal end (ab52473)

概述

  • 产品名称
    Anti-Ninein抗体- Aminoterminal end
    参阅全部 Ninein 一抗
  • 描述
    兔多克隆抗体to Ninein - Aminoterminal end
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human Ninein (N terminal).

  • 阳性对照
    • MCF7, MOLT-4, HOS, NIH3T3, HeLa and MCF-7 cell line extracts

性能

应用

Our Abpromise guarantee covers the use of ab52473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 200 kDa.

靶标

Anti-Ninein antibody - Aminoterminal end 图像

  • All lanes : Anti-Ninein antibody - Aminoterminal end (ab52473) (10 µl per 5 ml antibody dilution buffer for each mini-gel.)

    Lane 1 : HOS cell lysate
    Lane 2 : NIH3T3 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF-7 cell lysate
  • All lanes : Anti-Ninein antibody - Aminoterminal end (ab52473) at 1/500 dilution

    Lane 1 : MCF7 cell line extract
    Lane 2 : MOLT4 cell line extract

    Secondary
    HRP conjugated donkey anti-rabbit
    Developed using the ECL technique

    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,130 kDa. We are unsure as to the identity of these extra bands.Additional bands are observed only in hematopoietic cells (such as MOLT-4).

Anti-Ninein antibody - Aminoterminal end (ab52473)参考文献

This product has been referenced in:
  • Goldspink DA  et al. Ninein is essential for apico-basal microtubule formation and CLIP-170 facilitates its redeployment to non-centrosomal microtubule organizing centres. Open Biol 7:N/A (2017). Read more (PubMed: 28179500) »

See 1 Publication for this product

Product Wall

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Dog Cell (MDCK-2 kidney epithelial)
Specification
MDCK-2 kidney epithelial
Permeabilization
No
Fixative
Methanol
Username

Abcam user community

Verified customer

提交于 May 24 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mIMCD-3 kidney epithelial cell line)
Specification
mIMCD-3 kidney epithelial cell line
Fixative
Methanol
Permeabilization
Yes - NP40
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Mr. Benjamin Tyrrell

Verified customer

提交于 May 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (ARPE-19 cells)
Specification
ARPE-19 cells
Fixative
Methanol
Permeabilization
Yes - NP40
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Mr. Benjamin Tyrrell

Verified customer

提交于 May 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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