Cell line: NIH/3T3 (Mouse embryonic fibroblast).
Cells were cultured in medium supplemented with 50 ng/ml TPA (phorbol,
12-myristate, 13 acetate) for 2 hours at 37°C immediately prior to
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
NIH 3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition. This cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. It is therefore used for DNA transfection studies