概述

  • 产品名称Nifuroxazide
  • 描述
    JAK/STAT signaling inhibitor. Nitrofuran antibiotic.
  • 生物学描述JAK/STAT signaling inhibitor. Nitrofuran antibiotic. Inhibits JAK2 and Tyk2 phosphorylation and subsequent downstream STAT3 inhibition (EC50 = 3 μM). Antidiarrheal. Shows little effect on Akt, NF-κB, JAK1, MAPK and Src signaling.
  • 纯度> 98%

性能

    应用

    Our Abpromise guarantee covers the use of ab120951 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Nifuroxazide 图像

    • FOX-NY cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of nifuroxazide (ab120951). Decreased expression of Stat3 (phospho Y705) (ab76315) in FOX-NY cells correlates with an increase in nifuroxazide concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab76315 at 1/20000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    Nifuroxazide (ab120951)参考文献

    This product has been referenced in:
    • Fernandes MB  et al. Caco-2 cells cytotoxicity of nifuroxazide derivatives with potential activity against Methicillin-resistant Staphylococcus aureus (MRSA). Toxicol In Vitro 26:535-40 (2012). Read more (PubMed: 22285235) »
    • Nelson EA  et al. Nifuroxazide inhibits survival of multiple myeloma cells by directly inhibiting STAT3. Blood 112:5095-102 (2008). Read more (PubMed: 18824601) »

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