概述

  • 产品名称Anti-NIFK (phospho T234)抗体
    参阅全部 NIFK 一抗
  • 描述
    兔多克隆抗体to NIFK (phospho T234)
  • 特异性ab61110 detects endogenous levels of NIFK only when phosphorylated at threonine 234.
  • 经测试应用适用于: ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from human NIFK around the phosphorylation site of threonine 234 (G-P-TP-P-V).

  • 阳性对照
    • Human brain tissue and HuvEC cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆��克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab61110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/500.

靶标

  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
  • 细胞定位Nucleus > nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hNIFK antibody
    • MK67I_HUMAN antibody
    • MKI67 FHA Domain Interacting Nucleolar Phosphoprotein antibody
    • MKI67 FHA domain-interacting nucleolar phosphoprotein antibody
    • mki67ip antibody
    • NOPP34 antibody
    • Nucleolar phosphoprotein Nopp34 antibody
    • Nucleolar protein interacting with the FHA domain of pKI 67 antibody
    • Nucleolar protein interacting with the FHA domain of pKI-67 antibody
    • pKi67 antibody
    see all

Anti-NIFK (phospho T234) antibody 图像

  • ab61110 at a 1/50-1/100 dilution, staining human NIFK in brain tissue with (+) or without (-) immunising phospopeptide, using Immunohistochemistry, Paraffin embedded tissue
  • ab61110, at a 1/100-1/500 dilution, staining human NIFK in HuvEC cells with (+) or without (-) immunising phospopeptide, using Immunofluorescence

Anti-NIFK (phospho T234) antibody (ab61110)参考文献

This product has been referenced in:
  • Booth DG  et al. Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery. Elife 3:e01641 (2014). ICC/IF ; Human . Read more (PubMed: 24867636) »

See 1 Publication for this product

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