The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).
1/100 - 1/500.
1/50 - 1/100.
Contains 1 RRM (RNA recognition motif) domain.
Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
Nucleus > nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.