Anti-Niemann Pick C2抗体(ab92736)


  • 产品名称Anti-Niemann Pick C2抗体
    参阅全部 Niemann Pick C2 一抗
  • 描述
    兔多克隆抗体to Niemann Pick C2
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Niemann Pick C2.

    (Peptide available as ab93309.)

  • 阳性对照
    • This antibody gave a positive signal in human testis tissue lysate.



Our Abpromise guarantee covers the use of ab92736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa).


  • 功能May be involved in the regulation of the lipid composition of sperm membranes during the maturation in the epididymis.
  • 组织特异性Epididymis.
  • 疾病相关Defects in NPC2 are the cause of Niemann-Pick disease type C2 (NPDC2) [MIM:607625]. A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C2 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood.
  • 序列相似性Belongs to the NPC2 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EDDM1 antibody
    • Epididymal protein 1 antibody
    • Epididymal secretory protein antibody
    • Epididymal secretory protein E1 antibody
    • hE1 antibody
    • Human epididymis-specific protein 1 antibody
    • Niemann-Pick disease type C2 antibody
    • Niemann-Pick disease type C2 protein antibody
    • NPC intracellular cholesterol transporter 2 antibody
    • NPC2 antibody
    • NPC2_HUMAN antibody
    • Tissue specific secretory protein antibody
    see all

Anti-Niemann Pick C2 antibody 图像

  • Anti-Niemann Pick C2 antibody (ab92736) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)

    Exposure time : 10 minutes
  • IHC image of Niemann Pick C2 staining in human testis seminoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with 92736, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Niemann Pick C2 antibody (ab92736)参考文献

ab92736 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab92736.
Please use the links above to contact us or submit feedback about this product.