Anti-Niemann Pick C1抗体(ab55706)


  • 产品名称Anti-Niemann Pick C1抗体
    参阅全部 Niemann Pick C1 一抗
  • 描述
    小鼠单克隆抗体to Niemann Pick C1
  • 经测试应用适用于: WB, ICC, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原



Our Abpromise guarantee covers the use of ab55706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
ICC Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/10.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • 功能Involved in the intracellular trafficking of cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals.
  • 疾病相关Defects in NPC1 are the cause of Niemann-Pick disease type C1 (NPDC1) [MIM:257220]. A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C1 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood. An allelic variant of Niemann-Pick disease type C1 is found in people with Nova Scotia ancestry. Patients with the Nova Scotian clinical variant are less severely affected.
  • 序列相似性Belongs to the patched family.
    Contains 1 SSD (sterol-sensing) domain.
  • 结构域A cysteine-rich N-terminal domain and a C-terminal domain containing a di-leucine motif necessary for lysosomal targeting are critical for mobilization of cholesterol from lysosomes.
  • 翻译后修饰Glycosylated.
  • 细胞定位Late endosome membrane. Lysosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Niemann Pick C1 protein precursor antibody
    • Niemann Pick disease, type C1 antibody
    • Niemann-Pick C1 protein antibody
    • NPC antibody
    • NPC1 antibody
    • NPC1_HUMAN antibody
    see all

Anti-Niemann Pick C1 antibody 图像

  • Western blot against tagged recombinant protein immunogen using ab55706 Niemann Pick C1 antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa
  • ab55706 at 10 ug/ml staining Niemann Pick C1 in human A-431 cells by Immunocytochemistry/ Immunofluorescence.

  • IHC image of ab55706 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab55706, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing A431 cells stained with ab55706 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55706, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Niemann Pick C1 antibody (ab55706)参考文献

This product has been referenced in:
  • Field FJ  et al. TNF-alpha decreases ABCA1 expression and attenuates HDL cholesterol efflux in the human intestinal cell line Caco-2. J Lipid Res 51:1407-15 (2010). Read more (PubMed: 20103810) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry.

Unfortunately, I don't think that any of the we have in our catalog is suitable for the detection of the yeast Niemann-Pick type C-related protein 1 (http://www.unip...

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I spoke with regarding Lisa regarding ab3698863.

I don't believe I spoke with her about ab55706, however. What sort of trouble are you having with ab36983?

Thanks for the additional information!

Thank you for your reply. I have processed you request to receive free of charge replacement  vials of ab36983 and ab55706. Your new order number is *******. I hope that these replacement antibodies will be more successful.  If there is anything else I...

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Thank you for contacting Abcam. I am sorry that you have been having problems using both ab36983 and ab55706. Under our Abpromise both of these antibodies are guaranteed to work in western blot and ICC/IF and for ab36938, it has been tested on ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (macrophages)
Specification macrophages
Fixative Formaldehyde
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Feb 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1 cells)
Loading amount 30 µg
Specification THP-1 cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Nov 20 2009