概述

  • 产品名称Anti-Nidogen 2抗体
    参阅全部 Nidogen 2 一抗
  • 描述
    兔多克隆抗体to Nidogen 2
  • 经测试应用适用于: WB, ELISA, IHC-Fr, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant full length protein (Mouse).

性能

应用

Our Abpromise guarantee covers the use of ab14513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 153 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 23211522ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200. PubMed: 23409002

靶标

  • 功能Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.
  • 组织特异性Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle.
  • 序列相似性Contains 5 EGF-like domains.
    Contains 5 LDL-receptor class B repeats.
    Contains 1 NIDO domain.
    Contains 1 nidogen G2 beta-barrel domain.
    Contains 2 thyroglobulin type-1 domains.
  • 翻译后修饰Highly N- and O-glycosylated.
  • 细胞定位Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NID 2 antibody
    • NID-2 antibody
    • Nid2 antibody
    • NID2_HUMAN antibody
    • Nidogen-2 antibody
    • Nidogen2 antibody
    • Osteonidogen antibody
    see all

Anti-Nidogen 2 antibody (ab14513)参考文献

This product has been referenced in:
  • Hummitzsch K  et al. A new model of development of the mammalian ovary and follicles. PLoS One 8:e55578 (2013). ICC/IF ; Cow . Read more (PubMed: 23409002) »
  • Behrens DT  et al. The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens. J Biol Chem 287:18700-9 (2012). Read more (PubMed: 22493504) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (retinal tissue)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Heat mediated antigen retrieval solution pH: 6
Permeabilization Yes - 0.1% Tween 20
Specification retinal tissue
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Metanol 95% C/ Acético Glacial 5%
Username

Dr. Patricia Bogdanov

Verified customer

提交于 Jul 18 2016

Thank you for your enquiry. I can confirm that both ab14511 and ab14513 are sold as whole antiserum. As antibody concentration is usually determined by a protein assay, I am sorry it is not possible to provide a concentration for antibody in antiser...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"