重组Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1抗体[EP2067Y] (ab76159)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2067Y] to Nicotinic Acetylcholine Receptor beta/CHRNB1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1抗体[EP2067Y]
参阅全部 Nicotinic Acetylcholine Receptor beta/CHRNB1 一抗 -
描述
兔单克隆抗体[EP2067Y] to Nicotinic Acetylcholine Receptor beta/CHRNB1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human, mouse and rat brain lysates.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2067Y -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76159于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 57 kDa.
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说明 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 57 kDa. |
靶标
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功能
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
疾病相关
Defects in CHRNB1 are a cause of congenital myasthenic syndrome slow-channel type (SCCMS) [MIM:601462]. SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes.
Defects in CHRNB1 are a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (ACHRDCMS) [MIM:608931]. ACHRDCMS is a post-synaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. -
序列相似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-1/CHRNB1 sub-subfamily. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1140 Human
- Entrez Gene: 11443 Mouse
- Entrez Gene: 24261 Rat
- GenBank: NP_000738.2 Human
- Omim: 100710 Human
- SwissProt: P11230 Human
- SwissProt: P09690 Mouse
- SwissProt: P25109 Rat
see all -
别名
- Acetylcholine receptor protein beta chain precursor antibody
- Acetylcholine receptor subunit beta antibody
- ACHB_HUMAN antibody
see all
图片
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Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] (ab76159) at 1/1000 dilution + Human brain lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa -
Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] (ab76159) at 1/1000 dilution + Mouse brain lysate
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 5 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] (ab76159) at 1/1000 dilution + Rat brain lysate
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 5 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab76159 被引用在 2 文献中.
- Li B et al. Stability of motor endplates is greater in the biceps than in the interossei in a rat model of obstetric brachial plexus palsy. Neural Regen Res 15:1678-1685 (2020). PubMed: 32209772
- Kelly NA et al. Effects of aging and Parkinson's disease on motor unit remodeling: influence of resistance exercise training. J Appl Physiol (1985) 124:888-898 (2018). PubMed: 29357501