Anti-Nicotinic Acetylcholine Receptor beta 4抗体(ab41176)

概述

  • 产品名称Anti-Nicotinic Acetylcholine Receptor beta 4抗体
    参阅全部 Nicotinic Acetylcholine Receptor beta 4 一抗
  • 描述
    兔多克隆抗体to Nicotinic Acetylcholine Receptor beta 4
  • 特异性80 % cross reactivity with the full lenght Nicotinic ß4 receptor
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    EGPYAAQRD

    , corresponding to C terminal amino acids 490-498 of Human Nicotinic Acetylcholine Receptor beta 4 attached onto a carrier protein.

性能

应用

Our Abpromise guarantee covers the use of ab41176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ICC/IF 1/200.

靶标

  • 功能After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • 序列相似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-4/CHRNB4 sub-subfamily.
  • 细胞定位Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • acetylcholine receptor, nicotinic, beta 4 (neuronal) antibody
    • ACHB4_HUMAN antibody
    • AChR antibody
    • Cholinergic receptor nicotinic beta 4 antibody
    • Cholinergic receptor nicotinic beta polypeptide 4 antibody
    • cholinergic receptor, nicotinic, beta polypeptide 4 antibody
    • Chrnb4 antibody
    • Neuronal acetylcholine receptor subunit beta-4 antibody
    • Neuronal nicotinic receptor beta 4 subunit antibody
    see all

Anti-Nicotinic Acetylcholine Receptor beta 4 antibody 图像

  • ICC/IF image of ab41176 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41176, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Nicotinic Acetylcholine Receptor beta 4 antibody (ab41176)参考文献

ab41176 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab41176.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"