Anti-Nicotinic Acetylcholine Receptor alpha 7抗体(ab10096)

概述

  • 产品名称Anti-Nicotinic Acetylcholine Receptor alpha 7抗体
    参阅全部 Nicotinic Acetylcholine Receptor alpha 7 一抗
  • 描述
    兔多克隆抗体to Nicotinic Acetylcholine Receptor alpha 7
  • 特异性Several significant splice variants of the protein have been reported, but currently, we do not know, which variants are detected by this antibody.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH, selected from within the amino acid range 22-71 of Human Nicotinic Acetylcholine Receptor alpha 7. (Peptide available as ab101467.)

  • 阳性对照
    • HepG2 lysate and Mouse muscle lysate.

性能

应用

Our Abpromise guarantee covers the use of ab10096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Can be blocked with Human Nicotinic Acetylcholine Receptor alpha 7 peptide (ab101467). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
  • 序列相似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.
  • 细胞定位Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 7nAChR antibody
    • a7 nicotinic acetylcholine receptor antibody
    • acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
    • ACHA7_HUMAN antibody
    • AChR antibody
    • Acra7 antibody
    • alpha 7 neuronal nicotinic acetylcholine receptor antibody
    • alpha-7 nicotinic cholinergic receptor subunit antibody
    • alpha7 antibody
    • cholinergic receptor, neuronal nicotinic, alpha polypeptide 7 antibody
    • cholinergic receptor, nicotinic, alpha 7 (neuronal) antibody
    • cholinergic receptor, nicotinic, alpha 7 antibody
    • cholinergic receptor, nicotinic, alpha polypeptide 7 antibody
    • CHRNA7 antibody
    • CHRNA7-2 antibody
    • NACHRA7 antibody
    • neuronal acetylcholine receptor protein, alpha-7 chain antibody
    • Neuronal acetylcholine receptor subunit alpha-7 antibody
    see all

Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody 图像

  • Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab10096) at 2.5 µg/ml + HepG2 cell lysate

    Predicted band size : 56 kDa
  • NNK (NCI) (100 mg/kg) was administered to BALB/c mice. The mice were randomized into two groups; group one received the vehicle (PBS) (n = 8) and group two received nicotine (n = 8) by i.p. injection at a dose of 1 mg/kg three times a week for 28 weeks. Lungs from the mice were fixed in 10% neutral-buffered formalin before processing into paraffin blocks. Sections were subjected to microwave ‘antigen retrieval’ for 20 minutes on 70% power, with a 1 minute cooling period after every 5 minutes, in 0.01 M sodium citrate, pH 6.0. Slides were stained either with H&E alone or with ab10096 against α7 nAChR (1∶50 dilution). For color development the slides were treated with peroxidase substrate kit from Vector labs and developed using DAB as chromogen. After a final rinse in dH2O, sections were lightly counterstained in hematoxylin, dehydrated and mounted.



  • Predicted band size : 56 kDa
    WB using ab10096. Blots were incubated with ab10096 for at least 1 hour and for 30-90 minutes for the secondary antibody. 3% BSA and 10% FBS was used as blocking solution. anti- bungarotoxin was added in excess before precipitation in lanes 2 and 4. Nicotinic acetylcholine receptor alpha 7 precipitates as a double band (as shown by Drisdel and Green, 2000). The higher bands (79-122 kD) are probably dimeric and trimeric forms of the protein.  Review by Andreas Wiesner submitted 7 May 2004.
      WB using ab10096. Blots were incubated with ab10096 for at least 1 hour and for 30-90 minutes for the secondary antibody. 3% BSA and 10% FBS was used as blocking solution. anti- bungarotoxin was added in excess before precipitation in lanes 2 and 4. Nicotinic acetylcholine receptor alpha 7 precipitates as a double band (as shown by Drisdel and Green, 2000). The higher bands (79-122 kD) are probably dimeric and trimeric forms of the protein. Review by Andreas Wiesner submitted 7 May 200

Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab10096)参考文献

This product has been referenced in:
  • Lavezzi AM  et al. Possible role of the a7 nicotinic receptors in mediating nicotine's effect on developing lung - implications in unexplained human perinatal death. BMC Pulm Med 14:11 (2014). IHC-P ; Human . Read more (PubMed: 24484641) »
  • Horti AG  et al. 18F-ASEM, a radiolabeled antagonist for imaging the a7-nicotinic acetylcholine receptor with PET. J Nucl Med 55:672-7 (2014). Mouse . Read more (PubMed: 24556591) »

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4µg/mL · Temperature: 27°C
Username

Rita Machaalani

Verified customer

提交于 May 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (muscle)
Loading amount 10 µg
Specification muscle
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 18 2013

That is no problem. I hope they work well for you.

If I can be of any further assistance, please do not hesitate to let me know.

Until then, I wish you all the best with your research.

Thank you for your reply.

As we have no sequence for the proteins from Helix pomatia I cannot predict how likely the antibodies you would like to try will cross react. We would therefore not usually offer the testing discount scheme for this. ...

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Vielen Dank für ihren Anruf. Bitte entschuldigen Sie die Verzögerung. ich habe noch auf Immunogen Informationen aus dem herstellenden Labor gewartet.

Ich freue mich Ihnen bestätigen zu können, dass vier der fünf Abcam...

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Thank you for forwarding the complaint and for providing more information about your customer's protocol. Is this the same customer who is having problem with three of our antibodies at the same time? One of our collaborators has tested this antib...

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