Anti-Nicotinic Acetylcholine Receptor alpha 5抗体(ab26099)

概述

  • 产品名称
    Anti-Nicotinic Acetylcholine Receptor alpha 5抗体
    参阅全部 Nicotinic Acetylcholine Receptor alpha 5 一抗
  • 描述
    兔多克隆抗体to Nicotinic Acetylcholine Receptor alpha 5
  • 特异性
    ab26099 recognises Neuronal acetylcholine receptor protein, alpha-5 chain precursor(CHRNA5).
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Chicken, Cow, Dog, Chimpanzee
  • 免疫原

    A region within a synthetic peptide: LQNCSMKFGS WTYDGSQVDI ILEDQDVDKR DFFDNGEWEI VSATGSKGNR, corresponding to internal sequence amino acids 181-230 of Human Nicotinic Acetylcholine Receptor alpha 5

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab26099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2.5 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.

靶标

图片

  • Anti-Nicotinic Acetylcholine Receptor alpha 5 antibody (ab26099) at 2.5 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab26099 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15µg/mL · Temperature: 22°C
Antigen retrieval step
None
Sample
Rat Tissue sections (brain)
Specification
brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

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提交于 Jul 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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