重组Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4抗体[EPR4563(2)] (ab124832)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4563(2)] to Nicotinic Acetylcholine Receptor alpha 4/CHRNA4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4抗体[EPR4563(2)]
参阅全部 Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 一抗 -
描述
兔单克隆抗体[EPR4563(2)] to Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 aa 500-600. The exact sequence is proprietary.
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阳性对照
- SH-SY5Y, A673, C6 and Neuro-2a cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4563(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124832于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
靶标
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功能
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
疾病相关
Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. -
序列相似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1137 Human
- Entrez Gene: 11438 Mouse
- Entrez Gene: 25590 Rat
- Omim: 118504 Human
- SwissProt: P43681 Human
- SwissProt: O70174 Mouse
- SwissProt: P09483 Rat
- Unigene: 10734 Human
see all -
别名
- A4 nicotinic receptor antibody
- Acetylcholine receptor alpha 4 neural antibody
- Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
see all
图片
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All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] (ab124832) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : A673 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab124832 被引用在 5 文献中.
- Sun Z et al. Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of aO-Conotoxin GeXIVA. Mar Drugs 18:N/A (2020). PubMed: 31963558
- Liu Y et al. Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets. Mar Drugs 17:N/A (2019). PubMed: 31035425
- Chen SP et al. Involvement of hippocampal acetylcholinergic receptors in electroacupuncture analgesia in neuropathic pain rats. Behav Brain Funct 12:13 (2016). PubMed: 27068709
- Yin Y et al. Accumulation of human full-length tau induces degradation of nicotinic acetylcholine receptor a4 via activating calpain-2. Sci Rep 6:27283 (2016). WB . PubMed: 27277673
- Ciudad-Roberts A et al. Protracted treatment with MDMA induces heteromeric nicotinic receptor up-regulation in the rat brain: an autoradiography study. Prog Neuropsychopharmacol Biol Psychiatry 53:1-8 (2014). PubMed: 24614096