Anti-Nicotinic Acetylcholine Receptor alpha 4抗体(ab88239)

概述

  • 产品名称Anti-Nicotinic Acetylcholine Receptor alpha 4抗体
    参阅全部 Nicotinic Acetylcholine Receptor alpha 4 一抗
  • 描述
    兔多克隆抗体to Nicotinic Acetylcholine Receptor alpha 4
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human Nicotinic Acetylcholine Receptor alpha 4.

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: SW480 Whole Cell; HepG2 Whole Cell; HeLa Whole Cell; Jurkat Whole Cell; Human Brain Tissue; Rat Brain Tissue; Mouse Cerebellum Tissue; Rat Cerebellum Tissue.

性能

应用

Our Abpromise guarantee covers the use of ab88239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa).

靶标

  • 功能After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • 疾病相关Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
  • 序列相似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
  • 细胞定位Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A4 nicotinic receptor antibody
    • Acetylcholine receptor alpha 4 neural antibody
    • Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
    • ACH 4 antibody
    • ACH4 antibody
    • ACHA4_HUMAN antibody
    • AChR antibody
    • Acra 4 antibody
    • Acra4 antibody
    • Alpha4 nAChR antibody
    • BFNC antibody
    • Cholinergic receptor nicotinic alpha 4 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 4 antibody
    • CHRNA 4 antibody
    • CHRNA4 antibody
    • EBN 1 antibody
    • EBN antibody
    • EBN1 antibody
    • NACHR antibody
    • NACRA 4 antibody
    • NACRA4 antibody
    • Neuronal acetylcholine receptor subunit alpha-4 antibody
    • Neuronal nicotinic acetylcholine receptor alpha 4 subunit antibody
    see all

Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody 图像

  • All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab88239) at 1 µg/ml

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • ICC/IF image of ab88239 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88239, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
  • All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab88239) at 1 µg/ml

    Lane 1 : Cerebellum (Mouse) Tissue Lysate
    Lane 2 : Cerebellum (Rat) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 70 kDa


    Exposure time : 4 minutes

Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab88239)参考文献

ab88239 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab88239.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"