Anti-Nicotinic Acetylcholine Receptor alpha 4抗体(ab77356)

概述

  • 产品名称Anti-Nicotinic Acetylcholine Receptor alpha 4抗体
    参阅全部 Nicotinic Acetylcholine Receptor alpha 4 一抗
  • 描述
    山羊多克隆抗体to Nicotinic Acetylcholine Receptor alpha 4
  • 特异性ab77356 is not expected to cross-react with the similar alpha 2 subunit.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Chicken, Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    HVETRAHAEERLLKK

    , corresponding to internal sequence amino acids 29-43 of Human Nicotinic Acetylcholine Receptor alpha 4

  • 阳性对照
    • Rat Brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab77356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64,000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
    An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
    • 疾病相关Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
    • 序列相似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
    • 细胞定位Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • A4 nicotinic receptor antibody
      • Acetylcholine receptor alpha 4 neural antibody
      • Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
      • ACH 4 antibody
      • ACH4 antibody
      • ACHA4_HUMAN antibody
      • AChR antibody
      • Acra 4 antibody
      • Acra4 antibody
      • Alpha4 nAChR antibody
      • BFNC antibody
      • Cholinergic receptor nicotinic alpha 4 antibody
      • Cholinergic receptor nicotinic alpha polypeptide 4 antibody
      • CHRNA 4 antibody
      • CHRNA4 antibody
      • EBN 1 antibody
      • EBN antibody
      • EBN1 antibody
      • NACHR antibody
      • NACRA 4 antibody
      • NACRA4 antibody
      • Neuronal acetylcholine receptor subunit alpha-4 antibody
      • Neuronal nicotinic acetylcholine receptor alpha 4 subunit antibody
      see all

    Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody 图像

    • Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab77356) at 0.3 µg/ml + Rat Brain lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 70 kDa
      Observed band size : 70 kDa
      Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab77356)参考文献

    ab77356 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your inquiry.

    The molecular weight of the peptide was not determined.

    The molecular weight of the antibody is ca 150kDa.

    Molecular weight is not necessary to calculate the dilutions of the blocking peptide. C...

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    Vielen Dank für Ihren Anruf. Auf Ihre Anfrage hin haben wir das Blockpeptid für den Acetylcholin-Rezeptor alpha 4 (ab77356) in unseren Katalog aufgenommen. Es hat die Artikelnummer ab123011 und kostet 175 EUR plus Versandgebühren. Click here (or ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"