Anti-Nicotinic Acetylcholine Receptor alpha 4抗体(ab41172)


  • 产品名称
    Anti-Nicotinic Acetylcholine Receptor alpha 4抗体
    参阅全部 Nicotinic Acetylcholine Receptor alpha 4 一抗
  • 描述
    兔多克隆抗体to Nicotinic Acetylcholine Receptor alpha 4
  • 宿主
  • 特异性
    Antiserum Specificity Polypeptide % Cross Reactivity Nicotinic a4 receptor (620-627) 100 Nicotinic a4 receptor ~80 Nicotinic a3 receptor 0 Nicotinic a5 receptor 0 Nicotinic a7 receptor 0 Nicotinic ß2 receptor 0 Nicotinic ß3 receptor 0 Nicotinic ß4 receptor 0
  • 经测试应用
    适用于: WB, ICC/IF, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human, Chimpanzee, Pufferfish, Zebrafish, Snail
  • 免疫原

    Synthetic peptide:


    conjugated to KLH by a Cysteine residue linker, corresponding to C terminal amino acids 620-627 of Human Nicotinic Acetylcholine Receptor alpha 4



Our Abpromise guarantee covers the use of ab41172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/800. Predicted molecular weight: 70 kDa.Can be blocked with Human Nicotinic Acetylcholine Receptor alpha 4 peptide (ab99319). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ICC/IF 1/200.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/1000.


  • 功能
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • 疾病相关
    Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
  • 序列相似性
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
  • 细胞定位
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A4 nicotinic receptor antibody
    • Acetylcholine receptor alpha 4 neural antibody
    • Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
    • ACH 4 antibody
    • ACH4 antibody
    • ACHA4_HUMAN antibody
    • AChR antibody
    • Acra 4 antibody
    • Acra4 antibody
    • Alpha4 nAChR antibody
    • BFNC antibody
    • Cholinergic receptor nicotinic alpha 4 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 4 antibody
    • CHRNA 4 antibody
    • CHRNA4 antibody
    • EBN 1 antibody
    • EBN antibody
    • EBN1 antibody
    • NACHR antibody
    • NACRA 4 antibody
    • NACRA4 antibody
    • Neuronal acetylcholine receptor subunit alpha-4 antibody
    • Neuronal nicotinic acetylcholine receptor alpha 4 subunit antibody
    see all


  • All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab41172) at 1/800 dilution

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 70 kDa

  • ab41172 (1/250 dilution) staining Nicotinic Acetylcholine Receptor alpha 4 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and membrane compartment within the glia region of cells
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.


This product has been referenced in:
  • Chen N  et al. An acetylcholine-activated microcircuit drives temporal dynamics of cortical activity. Nat Neurosci 18:892-902 (2015). IHC . Read more (PubMed: 25915477) »

See 1 Publication for this product


Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 24°C
Snail (Helix pomatia) Tissue sections (brain)
Yes - TX

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Verified customer

提交于 Jun 27 2013

Thank you for your reply.

As we have no sequence for the proteins from Helix pomatia I cannot predict how likely the antibodies you would like to try will cross react. We would therefore not usually offer the testing discount scheme for this. ...

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Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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Thank you very much for your interest in our antibodies.

We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

If a customer would like to test an antibody in an application o...

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