兔多克隆抗体to Nicotinic Acetylcholine Receptor alpha 3
Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Nicotinic Acetylcholine Receptor alpha 3.
This antibody gave a positive signal in Human Spinal Cord tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily.
Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
Information by UniProt
Cholinergic receptor neuronal nicotinic alpha polypeptide 3 antibody
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3 antibody (ab110801)
Anti-Nicotinic Acetylcholine Receptor alpha 3 antibody (ab110801) at 1 µg/ml + Human spinal cord tissue lysate - total protein (
) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
Additional bands at :
51 kDa (possible cleavage fragment).
Exposure time :
has not yet been referenced specifically in any publications.
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