Anti-Nicotinic Acetylcholine Receptor alpha 3抗体(ab110801)

概述

  • 产品名称
    Anti-Nicotinic Acetylcholine Receptor alpha 3抗体
    参阅全部 Nicotinic Acetylcholine Receptor alpha 3 一抗
  • 描述
    兔多克隆抗体to Nicotinic Acetylcholine Receptor alpha 3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Nicotinic Acetylcholine Receptor alpha 3.

  • 阳性对照
    • This antibody gave a positive signal in Human Spinal Cord tissue lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab110801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

靶标

  • 功能
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • 序列相似性
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily.
  • 细胞定位
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACHA3_HUMAN antibody
    • AChR antibody
    • Cholinergic receptor neuronal nicotinic alpha polypeptide 3 antibody
    • Cholinergic receptor nicotinic alpha 3 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 3 antibody
    • CHRNA 3 antibody
    • CHRNA3 antibody
    • LNCR2 antibody
    • MGC104879 antibody
    • NACHRA 3 antibody
    • NACHRA3 antibody
    • Neuronal acetylcholine receptor protein alpha 3 chain precursor antibody
    • Neuronal acetylcholine receptor subunit alpha 3 antibody
    • Neuronal acetylcholine receptor subunit alpha-3 antibody
    • Neuronal nicotinic acetylcholine receptor alpha 3 subunit antibody
    • PAOD2 antibody
    see all

图片

  • Anti-Nicotinic Acetylcholine Receptor alpha 3 antibody (ab110801) at 1 µg/ml + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa
    Additional bands at: 51 kDa (possible cleavage fragment)


    Exposure time: 8 minutes


    The band observed at 51 kDa could potentially be a cleaved form of Nicotinic Acetylcholine Receptor alpha 3 due to the presence of a 31 amino acid signal peptide. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab110801 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

实验方案

文献

This product has been referenced in:
  • Armstrong LC  et al. High-Throughput Patch Clamp Screening in Human a6-Containing Nicotinic Acetylcholine Receptors. SLAS Discov 22:686-695 (2017). Read more (PubMed: 28298165) »

See 1 Publication for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab110801.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册