兔多克隆抗体to Nicotinic Acetylcholine Receptor alpha 3
Synthetic peptide corresponding to a region within N terminal amino acids 30-60 of Human Nicotinic Acetylcholine Receptor alpha 3, conjugated to KLH.
CEM cell line lysate; Human brain tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
This antibody is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 57 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily.
Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
Information by UniProt
Cholinergic receptor neuronal nicotinic alpha polypeptide 3 antibody
Western blot - Nicotinic Acetylcholine Receptor alpha 3 antibody (ab103956)
Anti-Nicotinic Acetylcholine Receptor alpha 3 antibody (ab103956) at 1/100 dilution + CEM cell line lysate at 35 µg
Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nicotinic Acetylcholine Receptor alpha 3 antibody (ab103956)
ab103956, at a 1/50 dilution, staining Nicotinic Acetylcholine Receptor alpha 3 in formalin fixed and paraffin embedded Human brain tissue by Immunochemistry. Developed using peroxidase conjugated secondary antibody and DAB staining.
This product has been referenced in:
et al. CHRNA3 polymorphism modifies lung adenocarcinoma risk in the Chinese Han population. Int J Mol Sci 15:5446-57 (2014).
Read more (PubMed: 24686516) »
et al. Gamma-amino butyric acid (GABA) prevents the induction of nicotinic receptor-regulated signaling by chronic ethanol in pancreatic cancer cells and normal duct epithelia. Cancer Prev Res (Phila) 6:139-48 (2013).
Read more (PubMed: 23213073) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"