Anti-NHEDC1抗体(ab106124)

概述

  • 产品名称Anti-NHEDC1抗体
    参阅全部 NHEDC1 一抗
  • 描述
    兔多克隆抗体to NHEDC1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MHTTESKNEH LEDENFQTST TPQSLIDPNN TAHEETKTVL SDTEEIKPQT) of Human NHEDC1 (NP_001094344).

  • 阳性对照
    • Human fetal brain cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab106124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 组织特异性Expressed only in the testis.
  • 序列相似性Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1700094G20Rik antibody
    • 4933424B12Rik antibody
    • 4933425K02Rik antibody
    • AV258602 antibody
    • MGC131641 antibody
    • Na(+)/H(+) exchanger-like domain-containing protein 1 antibody
    • NHDC1_HUMAN antibody
    • NHE domain-containing protein 1 antibody
    • Nhedc1 antibody
    • Sodium/hydrogen exchanger-like domain-containing protein 1 antibody
    see all

Anti-NHEDC1 antibody 图像

  • Anti-NHEDC1 antibody (ab106124) at 0.5 µg/ml + Human fetal brain cell lysate at 10 µg

    Predicted band size : 56 kDa

Anti-NHEDC1 antibody (ab106124)参考文献

ab106124 has not yet been referenced specifically in any publications.

Product Wall

Thank you for keeping us updated on your results. I am sorry to hear that the secondary antibody is gives non-specific bands. I'm assuming that you ran a blot without the primary antibody, and that is how you know the extra bands are from the secondary...

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Thank you for your reply. Regarding the sample buffer, we used the following:  0.05M Tris-HCl, pH6.8, 0.1M DTT, 2%SDS, 10% glycerol, 0.1% bromophenol blue Although slightly, different, your buffer would be fine as well. Samples were then heated...

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Thank you for contacting Abcam regarding ab106124. Please find the WB protocol used to validate this antibody below: - Prepare 12% gel - Load 10ug human fetal brain cell lysate - Transfer protein to PVDF membrane (we used Bio-Rad Trans-Blot Ce...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"