RabMAb

Anti-NGF抗体[EP1320Y] (ab52918)

概述

  • 产品名称Anti-NGF抗体[EP1320Y]
    参阅全部 NGF 一抗
  • 描述
    兔单克隆抗体[EP1320Y] to NGF
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属���应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NGF aa 200-300.
    (Peptide available as ab154297)

  • 阳性对照
    • Human brain, fetal thyroid tissue.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab52918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/300.
WB 1/1000. Predicted molecular weight: 27 kDa.Can be blocked with NGF peptide (ab154297).

For unpurified, use 1/500.

IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

靶标

  • 功能Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
  • 疾病相关Neuropathy, hereditary sensory and autonomic, 5
  • 序列相似性Belongs to the NGF-beta family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta nerve growth factor antibody
    • Beta NGF antibody
    • Beta-nerve growth factor antibody
    • Beta-NGF antibody
    • HSAN5 antibody
    • MGC161426 antibody
    • MGC161428 antibody
    • Nerve growth factor (beta polypeptide) antibody
    • Nerve growth factor antibody
    • Nerve growth factor beta antibody
    • Nerve growth factor beta polypeptide antibody
    • Nerve growth factor beta subunit antibody
    • NGF antibody
    • NGF_HUMAN antibody
    • NGFB antibody
    • NID67 antibody
    see all

Anti-NGF antibody [EP1320Y] 图像

  • All lanes : Anti-NGF antibody [EP1320Y] (ab52918) at 1/1000 dilution (purified)

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal thymus lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 27 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Anti-NGF antibody [EP1320Y] (ab52918) at 1/1000 dilution (purified) + Mouse thyroid lysate at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 27 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Anti-NGF antibody [EP1320Y] (ab52918) at 1/2000 dilution (purified) + Rat thyroid lysate at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 27 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab52918 at a working dilution of 1 in 250. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunofluorescence staining of U87-MG cells with purified ab52918 at a working dilution of 1 in 300, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab52918 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

  • Anti-NGF antibody [EP1320Y] (ab52918) at 1/500 dilution (unpurified) + fetal thyroid tissue at 10 µg

    Secondary
    Goat anti rabbit IgG HRP antibody at 1/2000 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
  • Immunohistochemical staining of paraffin embedded human brain using unpurified b52918 at 1/50-1/100 dilution.

    Ab52918 at 1/50-1/100 dilution staining human brain; paraffin embedded.
  • Immunohistochemical staining of NGF mouse tissue sections (formalin/ PFA-fixed paraffin-embedded tissue sections) with unpurified ab52918. The sections were formaldehyde fixed, subjected to heat mediated antigen retrieval and blocked for 10 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with the sample for 1 hour at 25°C. An HRP polymer anti-rabbit IgG system was used undiluted, as the secondary antibody.

     

    See Abreview

Anti-NGF antibody [EP1320Y] (ab52918)参考文献

ab52918 has not yet been referenced specifically in any publications.

Product Wall

Product ab154297 can be used as the blocking peptide with ab52918.

Thank you for contacting us.

I am happy to confirm the blocking peptide for Ab52918 has now been added to our catalogue under reference ab154297.

I’d like to bring your attention to an offer we are running throughout November....

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Thank you for your enquiry.

I can confirm that many of our antibodies are supplied in purified format, and we can provide the concentration forthese.

However, unpurified antibodies, such as those sold as whole antiserum, ascites o...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse ear)
Specification Mouse ear
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako S2375 High pH HIER
Permeabilization No
Blocking step (agent) for 10 minute(s) · Concentration: 100% · Temperature: RT°C
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提交于 May 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"