Anti-NGF抗体[EP1318Y] (ab68151)


  • 产品名称Anti-NGF抗体[EP1318Y]
    参阅全部 NGF 一抗
  • 描述
    兔单克隆抗体[EP1318Y] to NGF
  • 特异性This antibody recognizes pro-NGF.
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human NGF-beta precursor (pro-NGF).

  • 阳性对照
    • Human pro-NGF recombinant protein
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.



Our Abpromise guarantee covers the use of ab68151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 27 kDa.

For unpurified use at 1/1000 - 1/5000.

Flow Cyt 1/200 - 1/280.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
    • 疾病相关Neuropathy, hereditary sensory and autonomic, 5
    • 序列相似性Belongs to the NGF-beta family.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Beta nerve growth factor antibody
      • Beta NGF antibody
      • Beta-nerve growth factor antibody
      • Beta-NGF antibody
      • HSAN5 antibody
      • MGC161426 antibody
      • MGC161428 antibody
      • Nerve growth factor (beta polypeptide) antibody
      • Nerve growth factor antibody
      • Nerve growth factor beta antibody
      • Nerve growth factor beta polypeptide antibody
      • Nerve growth factor beta subunit antibody
      • NGF antibody
      • NGF_HUMAN antibody
      • NGFB antibody
      • NID67 antibody
      see all

    Anti-NGF antibody [EP1318Y] 图像

    • Overlay histogram showing SH-SY5Y cells stained with ab68151 (red line) at 1/280 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)

    • Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Rat brain lysate at 10 µg

      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size : 27 kDa
    • Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Human fetal brain lysate at 10 µg

      Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

      Predicted band size : 27 kDa
    • All lanes : Anti-NGF antibody [EP1318Y] (ab68151) at 1/5000 dilution (unpurified)

      Lane 1 : 10ng human pro-NGF recombinant protein
      Lane 2 : 5ng human pro-NGF recombinant protein
      Lane 3 : 1ng human pro-NGF recombinant protein
      Lane 4 : 0.5ng human pro-NGF recombinant protein

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 27 kDa
      Observed band size : 27 kDa
      Additional bands at : 13 kDa,20 kDa. We are unsure as to the identity of these extra bands.

    Anti-NGF antibody [EP1318Y] (ab68151)参考文献

    ab68151 has not yet been referenced specifically in any publications.

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