重组Anti-proNGF抗体[EP1318Y] (ab68151)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1318Y] to proNGF
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-proNGF抗体[EP1318Y]
参阅全部 proNGF 一抗 -
描述
兔单克隆抗体[EP1318Y] to proNGF -
宿主
Rabbit -
特异性
This antibody recognizes pro-NGF and can work in tissues rather than cell lines for WB.
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经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: IHC-P -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse and Rat brain lysates. Mouse, Rat and Human cerebellum lysates. Human pro-NGF recombinant protein lysates. Flow Cyt (Intra): SH-SY5Y cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1318Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68151于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/200 - 1/280.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 27 kDa).
For unpurified use at 1/1000 - 1/5000. |
说明 |
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Flow Cyt (Intra)
1/200 - 1/280. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 27 kDa). For unpurified use at 1/1000 - 1/5000. |
靶标
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功能
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). -
疾病相关
Neuropathy, hereditary sensory and autonomic, 5 -
序列相似性
Belongs to the NGF-beta family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4803 Human
- SwissProt: P01138 Human
- SwissProt: P01139 Mouse
- SwissProt: P25427 Rat
- Unigene: 2561 Human
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别名
- Beta-nerve growth factor antibody
- Beta-NGF antibody
- NGF antibody
see all
图片
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Overlay histogram showing SH-SY5Y cells stained with ab68151 (red line) at 1/280 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)
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All lanes : Anti-proNGF antibody [EP1318Y] (ab68151) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human cerebellum lysate
Lane 4 : Mouse cerebellum lysate
Lane 5 : Rat cerebellum lysate
Lane 6 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 7 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 8 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsCompared with ab68151, ab52918 has a higher affinity in positive samples.
The positive cell lines (Lanes 7-9) are consistent with the literature: PMID:12944907, Fig1.
Blocking buffer: 5% NFDM/TBST.
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Anti-proNGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Rat brain tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 27 kDa -
All lanes : Anti-proNGF antibody [EP1318Y] (ab68151) at 1/5000 dilution (unpurified)
Lane 1 : 10ng human pro-NGF recombinant protein
Lane 2 : 5ng human pro-NGF recombinant protein
Lane 3 : 1ng human pro-NGF recombinant protein
Lane 4 : 0.5ng human pro-NGF recombinant protein
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
Additional bands at: 13 kDa, 20 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab68151 被引用在 4 文献中.
- Sun NY et al. Astragaloside-IV modulates NGF-induced osteoblast differentiation via the GSK3β/β-catenin signalling pathway. Mol Med Rep 23:N/A (2021). PubMed: 33179111
- Piovesana R et al. Muscarinic receptors modulate Nerve Growth Factor production in rat Schwann-like adipose-derived stem cells and in Schwann cells. Sci Rep 10:7159 (2020). PubMed: 32346125
- Protto V et al. Electroacupuncture in rats normalizes the diabetes-induced alterations in the septo-hippocampal cholinergic system. Hippocampus N/A:N/A (2019). PubMed: 30870587
- Cai H et al. Neuroprotective effects of bajijiasu against cognitive impairment induced by amyloid-ß in APP/PS1 mice. Oncotarget 8:92621-92634 (2017). PubMed: 29190943