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Our Abpromise guarantee covers the use of ab16161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
|ELISA||Use a concentration of 10 µg/ml. Can be paired for ELISA with Mouse monoclonal [AS18] to NGF (Biotin) (ab16160). (For coating in a two site ELISA with biotin conjugated NGF antibody for mouse NGF). Two site ELISA.|
|Neutralising||Use at an assay dependent dilution. This antibody has been used to neutralize > 90% human NGF bioactivity on PC12 cells at a 200 fold molar excess.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"