Highly pure (>98%) recombinant hNGFb (human Nerve Growth Factor beta).
Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 0.5 µg/ml, allows the detection of 0.2 - 0.4 ng/well of recombinant hNGFb.
WB: Use at a concentration of 0.1 - 0.2 µg/ml, detection limit for recombinant hNGFb is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neut: To yield one-half maximal inhibition [ND50] of the biological activity of NGF (1 ng/ml), a concentration of 0.05-0.08 µg/ml of this antibody is required.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
Ferrari MP et al. Safety and pharmacokinetics of escalating doses of human recombinant nerve growth factor eye drops in a double-masked, randomized clinical trial. BioDrugs28:275-83 (2014).
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