Anti-NG2抗体[LHM 2] (ab20156)


  • 产品名称Anti-NG2抗体[LHM 2]
    参阅全部 NG2 一抗
  • 描述
    小鼠单克隆抗体[LHM 2] to NG2
  • 经测试应用适用于: IHC-FoFr, WB, IP, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human - a 375P cell crude extract).

  • 常规说明The LHM2 antibody is a very good marker for melanoma (>90% stained). LHM2 also stains basal cell carcinomas and subpopulation of basal keratinocytes. LHM2 can be used as a diagnostic panel for immunopathology of malignant melanoma and potential candidates for immunoimaging and immunotherapy of disseminated melanoma. The originators of this antibody report that the MCSP is a cell surface marker specific for epidermal stem cells (J. Invest. Dermatol. in press). Potential applications of the LHM2 antibody include use for separation/isolation of epidermal stem cells, detection of stem cells in skin biopsies or epithelial cell cultures and optimisation of culture conditions to retain epidermal stem cell population in in vitro cultures.



Our Abpromise guarantee covers the use of ab20156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 21970342
WB Use at an assay dependent concentration. Predicted molecular weight: 240 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 功能Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
  • 组织特异性Detected only in malignant melanoma cells.
  • 序列相似性Contains 15 CSPG (NG2) repeats.
    Contains 2 laminin G-like domains.
  • 翻译后修饰O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
    Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
  • 细胞定位Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4732461B14Rik antibody
    • AN2 antibody
    • AN2 proteoglycan antibody
    • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
    • Chondroitin sulfate proteoglycan 4 antibody
    • Chondroitin sulfate proteoglycan NG2 antibody
    • Cspg4 antibody
    • Cspg4 chondroitin sulfate proteoglycan 4 antibody
    • CSPG4_HUMAN antibody
    • HMW-MAA antibody
    • HSN tumor-specific antigen antibody
    • Kiaa4232 antibody
    • MCSP antibody
    • MCSPG antibody
    • MEL-CSPG antibody
    • Melanoma chondroitin sulfate proteoglycan antibody
    • Melanoma-associated chondroitin sulfate proteoglycan antibody
    • MELCSPG antibody
    • MSK16 antibody
    • NG2 antibody
    see all

Anti-NG2 antibody [LHM 2] (ab20156)参考文献

This product has been referenced in:
  • Somaiah N  et al. The relationship between homologous recombination repair and the sensitivity of human epidermis to the size of daily doses over a 5-week course of breast radiotherapy. Clin Cancer Res 18:5479-88 (2012). IHC-P ; Human . Read more (PubMed: 22855580) »
  • Hyldahl RD  et al. Activation of nuclear factor-?B following muscle eccentric contractions in humans is localized primarily to skeletal muscle-residing pericytes. FASEB J 25:2956-66 (2011). IHC-P ; Human . Read more (PubMed: 21602448) »

See all 9 Publications for this product

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Indeed, you are right. The three antibodies, ab78284, ab83508 and ab20156, are the only antibodies against NG2 which have been tested in flow cytometry and on human cells. Of those three, the ab20156 is the only on...

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DISCOUNT CODE for AB85308: Expiration date: I am very pleased to hear you would like to accept our offer and test ab20156 and/or ab85308 in IHC on rabbit. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem thi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 3%

Dr. Sonja Muffler

Verified customer

提交于 Dec 14 2006

The antibody was tested in IHC on keratinocytes in the epidermis in the paper Ghali L et al. Epidermal and hair follicle progenitor cells express melanoma-associated chondroitin sulfate proteoglycan core protein. J Invest Dermatol 122:433-42 (2004). Pu...

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