概述

  • 产品名称Anti-NG2抗体[9.2.27]
    参阅全部 NG2 一抗
  • 描述
    小鼠单克隆抗体[9.2.27] to NG2
  • 特异性Recognizes mature NG2 core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa (Bumoi et al., 1984).
  • 经测试应用适用于: Electron Microscopy, Blocking, IP, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin SepharoseTM (Morgan et al., 1981).

  • 阳性对照
    • Human melanoma, glioma and proliferating brain endothelial cells.

性能

应用

Our Abpromise guarantee covers the use of ab78284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Electron Microscopy
Blocking
IP
Flow Cyt
ICC/IF
  • 应用说明BL: Use at an assay dependent dilution. Has been used for functional block of melanoma cell spreading, glioma cell proliferation and tumor growth in nude mice.
    Immunoelectron microscopy: Use at an assay dependent dilution.
    Flow Cyt: Use 1-2µg for 106 cells.
    Immunofluorescence microscopy of cells in culture: Use at an assay dependent dilution.
    IP: Use 1µg for 106 cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
    • 组织特异性Detected only in malignant melanoma cells.
    • 序列相似性Contains 15 CSPG (NG2) repeats.
      Contains 2 laminin G-like domains.
    • 翻译后修饰O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
      Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
    • 细胞定位Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4732461B14Rik antibody
      • AN2 antibody
      • AN2 proteoglycan antibody
      • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
      • Chondroitin sulfate proteoglycan 4 antibody
      • Chondroitin sulfate proteoglycan NG2 antibody
      • Cspg4 antibody
      • Cspg4 chondroitin sulfate proteoglycan 4 antibody
      • CSPG4_HUMAN antibody
      • HMW-MAA antibody
      • HSN tumor-specific antigen antibody
      • Kiaa4232 antibody
      • MCSP antibody
      • MCSPG antibody
      • MEL-CSPG antibody
      • Melanoma chondroitin sulfate proteoglycan antibody
      • Melanoma-associated chondroitin sulfate proteoglycan antibody
      • MELCSPG antibody
      • MSK16 antibody
      • NG2 antibody
      see all

    Anti-NG2 antibody [9.2.27] (ab78284)参考文献

    This product has been referenced in:
    • Chen SY  et al. Isolation and characterization of mesenchymal progenitor cells from human orbital adipose tissue. Invest Ophthalmol Vis Sci 55:4842-52 (2014). IF ; Human . Read more (PubMed: 24994870) »
    • Schrappe M  et al. Long-term growth suppression of human glioma xenografts by chemoimmunoconjugates of 4-desacetylvinblastine-3-carboxyhydrazide and monoclonal antibody 9.2.27. Cancer Res 52:3838-44 (1992). ICC/IF, Blocking ; Human . Read more (PubMed: 1617657) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your inquiry.

    Indeed, you are right. The three antibodies, ab78284, ab83508 and ab20156, are the only antibodies against NG2 which have been tested in flow cytometry and on human cells. Of those three, the ab20156 is the only on...

    Read More

    Merci de nous avoir contactés.

    Nous n'avons malheureusement pas d'anticorps anti-NG2 conjugué à un fluorochrome. Nous avons par contre des kits de conjugaison simples et rapides à utiliser permettant le couplage d'...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"