Anti-NG2抗体[132.38] (ab50009)

概述

  • 产品名称
    Anti-NG2抗体[132.38]
    参阅全部 NG2 一抗
  • 描述
    小鼠单克隆抗体[132.38] to NG2
  • 经测试应用
    适用于: WB, IP, ELISA, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Cell extract of HEK293 cells expressing the D3 domain of rat NG2, corresponding to amino acids 1592-2222.

  • 阳性对照
    • This antibody gave a positive signal in the following rat tissue lysates: spinal cord; whole brain; hippocampus.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    This antibody is derived from the hybridoma 132.38 produced by the fusion of mouse myeloma cells (P3XAg8.653) and splenocytes from BALB/c mice.

    The Protocols tab contains a Mouse on Mouse staining protocol with recommendations when using a mouse monoclonal antibody to stain mouse tissues and tips for reducing background.

性能

应用

Our Abpromise guarantee covers the use of ab50009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa).

Abcam recommends using BSA as the blocking agent.

IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/200. PubMed: 18974869
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 功能
    Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
  • 组织特异性
    Detected only in malignant melanoma cells.
  • 序列相似性
    Contains 15 CSPG (NG2) repeats.
    Contains 2 laminin G-like domains.
  • 翻译后修饰
    O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
    Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
  • 细胞定位
    Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4732461B14Rik antibody
    • AN2 antibody
    • AN2 proteoglycan antibody
    • Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
    • Chondroitin sulfate proteoglycan 4 antibody
    • Chondroitin sulfate proteoglycan NG2 antibody
    • Cspg4 antibody
    • Cspg4 chondroitin sulfate proteoglycan 4 antibody
    • CSPG4_HUMAN antibody
    • HMW-MAA antibody
    • HSN tumor-specific antigen antibody
    • Kiaa4232 antibody
    • MCSP antibody
    • MCSPG antibody
    • MEL-CSPG antibody
    • Melanoma chondroitin sulfate proteoglycan antibody
    • Melanoma-associated chondroitin sulfate proteoglycan antibody
    • MELCSPG antibody
    • MSK16 antibody
    • NG2 antibody
    see all

Anti-NG2 antibody [132.38] 图像

  • ab76897 staining NG2 in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/250) for 16 hours at 4°C. An Alexa Fluor®488 -conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab50009 staining NG2 in an immunoreactive cell (rat hippocampal) by Immunohistochemistry (PFA fixed).
    Brains were postfixed (2–6 hours) in 4% PFA and placed in 30% sucrose (2 days) and sectioned (20–30 µm) with a cryostat. ab50009 was used at a 1/200 dilution. A goat anti-mouse-Alexa 594, 1/500, was used as the secondary antibody.
  • All lanes : Anti-NG2 antibody [132.38] (ab50009) at 1 µg/ml

    Lane 1 : Rat Brain Tissue Lysate
    Lane 2 : Rat Spinal Cord Tissue Lysate
    Lane 3 : Rat Hippocampus Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 251 kDa
    Observed band size : 270 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa (possible non-specific binding),41 kDa (possible non-specific binding).

    Exposure time : 20 minutes

Anti-NG2 antibody [132.38] (ab50009)参考文献

This product has been referenced in:
  • Zhao W  et al. Mechanisms responsible for the inhibitory effects of epothilone B on scar formation after spinal cord injury. Neural Regen Res 12:478-485 (2017). IHC ; Rat . Read more (PubMed: 28469665) »
  • Raha-Chowdhury R  et al. Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci 16:24 (2015). IHC . Read more (PubMed: 25896789) »

See all 8 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
Permeabilization
Yes - 1% Triton-X
Specification
brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formalin
Username

Abcam user community

Verified customer

提交于 Jan 24 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - PBSTx 3x5mn RT
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Fixative
Paraformaldehyde
Username

Yacine Tensaouti

Verified customer

提交于 Aug 02 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step
None
Sample
Rat Tissue sections (brain)
Specification
brain
Permeabilization
Yes - 0.3% Triton-X
Fixative
Paraformaldehyde
Username

Mrs. Olga Cravetchi

Verified customer

提交于 Mar 02 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 23 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue (Brain section)
Specification
Brain section
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Feb 18 2013

Merci de ces précisions.

Eventuellement une autre fixation pourrait vous donner des meilleurs résultats, car la fixation acétone/méthanol fonctionne par précipitation et non pas crosslinking comme la PFA. Comme par contre nous avons des donn...

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Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolée d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab50009.
Nous avons eu un autre retour négative dernièrement sur c...

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Thank you for contacting us and reporting the problems you have been experienced in using Anti-NG2 antibody [132.38] (ab50009).

The details provided by you will enable us to investigate this case and will provide us with vital information for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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