Anti-NFYB抗体[NFYBA7] (ab51353)

概述

  • 产品名称
    Anti-NFYB抗体[NFYBA7]
    参阅全部 NFYB 一抗
  • 描述
    小鼠单克隆抗体[NFYBA7] to NFYB
  • 经测试应用
    适用于: WB, Dot blotmore details
  • 种属反应性
    与反应: Recombinant fragment
    预测可用于: Human
  • 免疫原

    Recombinant full length protein (Internal sequence).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
  • 克隆
    单克隆
  • 克隆编号
    NFYBA7
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.
Dot blot Use at an assay dependent dilution.

靶标

  • 功能
    Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • 序列相似性
    Belongs to the NFYB/HAP3 subunit family.
  • 结构域
    Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAAT box DNA binding protein subunit B antibody
    • CAAT box DNA-binding protein subunit B antibody
    • CBF A antibody
    • CBF B antibody
    • CCAAT binding transcription factor subunit A antibody
    • HAP 3 antibody
    • HAP3 antibody
    • NF Y protein chain B antibody
    • NF YB antibody
    • NF-YB antibody
    • NFYB antibody
    • NFYB_HUMAN antibody
    • Nuclear transcription factor Y beta antibody
    • Nuclear transcription factor Y subunit B antibody
    • Nuclear transcription factor Y subunit beta antibody
    • Transcription factor NF Y B subunit antibody
    see all

图片

  • Anti-NFYB antibody [NFYBA7] (ab51353) + immunized recombinant protein

    Predicted band size : 23 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,30 kDa. We are unsure as to the identity of these extra bands.
  • CBB staining of imunized recombinant protein

实验方案

文献

ab51353 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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