概述

  • 产品名称Anti-NFYB抗体
    参阅全部 NFYB 一抗
  • 描述
    兔多克隆抗体to NFYB
  • 特异性Minimal reaction was observed by WB against the 35 kDa A subunit of NF-Y
  • 经测试应用适用于: WB, IP, ELISA, EMSAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). The sequence is: QQI SGV QQI QFS

  • 阳性对照
    • CHO-7 cells that were cultured in the presence of cholesterol.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab6559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Predicted molecular weight: 23 kDa.
IP Use at an assay dependent dilution.
ELISA 1/4000.
EMSA Use 2µl for 104 cells.

靶标

  • 功能Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • 序列相似性Belongs to the NFYB/HAP3 subunit family.
  • 结构域Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAAT box DNA binding protein subunit B antibody
    • CAAT box DNA-binding protein subunit B antibody
    • CBF A antibody
    • CBF B antibody
    • CCAAT binding transcription factor subunit A antibody
    • HAP 3 antibody
    • HAP3 antibody
    • NF Y protein chain B antibody
    • NF YB antibody
    • NF-YB antibody
    • NFYB antibody
    • NFYB_HUMAN antibody
    • Nuclear transcription factor Y beta antibody
    • Nuclear transcription factor Y subunit B antibody
    • Nuclear transcription factor Y subunit beta antibody
    • Transcription factor NF Y B subunit antibody
    see all

Anti-NFYB antibody 图像

  • EMSA was performed with a double-stranded, biotin-labeled oligonucleotide in the presence of murine ES cell nuclear extracts and increasing amounts of anti-NFYB antibody (ab6559; 2 µg or 6 µg) or unrelated antibody.

Anti-NFYB antibody (ab6559)参考文献

This product has been referenced in:
  • Huang Y  et al. Phospho-?Np63a is a key regulator of the cisplatin-induced microRNAome in cancer cells. Cell Death Differ 18:1220-30 (2011). WB ; Human . Read more (PubMed: 21274007) »
  • Grskovic M  et al. Systematic identification of cis-regulatory sequences active in mouse and human embryonic stem cells. PLoS Genet 3:e145 (2007). EMSA ; Mouse . Read more (PubMed: 17784790) »

See all 2 Publications for this product

Product Wall

I'm sorry to hear you are having problems with ab6559. The protein NF-YB is know to function as a heterotrimeric transcription factor when bound to NF-YA and NF-YC. It is therefore possible that the large protein observed is made of NF-YB and other ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"