Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish
Recombinant fragment, corresponding to a region within amino acids 33-157 of Human NFYB.
- HeLa nuclear cell lysate; HeLa cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 23 kDa.
||1/100 - 1/500.
Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
Belongs to the NFYB/HAP3 subunit family.
Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.
Information by UniProt
- CAAT box DNA binding protein subunit B antibody
- CAAT box DNA-binding protein subunit B antibody
- CBF A antibody
Western blot - NFYB antibody (ab111577)
Anti-NFYB antibody (ab111577) at 1/1000 dilution + HeLa nuclear lysate at 30 µg
Predicted band size: 23 kDa
Immunocytochemistry/ Immunofluorescence - NFYB antibody (ab111577)
ab111577 at 1/500 dilution staining NFYB in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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