概述

  • 产品名称
  • 描述
    兔多克隆抗体to NFYA
  • 特异性
    No reaction occurs with B subunit.
  • 经测试应用
    适用于: WB, IP, ELISA, EMSAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product was prepared from monospecific antiserum by delipidation and defibrination.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 39 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
EMSA Use at an assay dependent dilution.
1-2 ul/assay.

靶标

  • 功能
    Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
  • 序列相似性
    Belongs to the NFYA/HAP2 subunit family.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAAT box DNA binding protein subunit A antibody
    • CAAT box DNA-binding protein subunit A antibody
    • CBF A antibody
    • CBF B antibody
    • CBFA antibody
    • CBFB antibody
    • CCAAT binding transcription factor subunit B antibody
    • FLJ11236 antibody
    • HAP 2 antibody
    • HAP2 antibody
    • HAP2 CCAAT binding protein antibody
    • NF YA antibody
    • NF-YA antibody
    • NFY protein chain A antibody
    • NFYA antibody
    • NFYA_HUMAN antibody
    • NUCLEAR FACTOR BINDING TO Y BOX OF HLA GENES antibody
    • Nuclear transcription factor Y alpha antibody
    • Nuclear transcription factor Y antibody
    • Nuclear transcription factor Y subunit A antibody
    • Nuclear transcription factor Y subunit alpha antibody
    • Sez10 antibody
    • Transcription factor NF Y A subunit antibody
    see all

Anti-NFYA antibody 图像

  • Anti-NFYA antibody (ab6558) at 1/500 dilution + CaPan1 whole cell lysate at 50 µg

    Secondary
    HRP-conjugated Donkey anti-Rabbit. at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 40 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview.

    See Abreview

Anti-NFYA antibody (ab6558)参考文献

This product has been referenced in:
  • Ma H  et al. ZHX2 enhances the cytotoxicity of chemotherapeutic drugs in liver tumor cells by repressing MDR1 via interfering with NF-YA. Oncotarget 6:1049-63 (2015). IP, ChIP ; Human . Read more (PubMed: 25473899) »
  • Lagadec C  et al. The RNA-binding protein Musashi-1 regulates proteasome subunit expression in breast cancer- and glioma-initiating cells. Stem Cells 32:135-44 (2014). Read more (PubMed: 24022895) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (CaPan1)
Loading amount
50 µg
Specification
CaPan1
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册