概述

  • 产品名称Anti-NFX1抗体
    参阅全部 NFX1 一抗
  • 描述
    兔多克隆抗体to NFX1
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 1071-1120 (TLTGVLEREM QARPPPPIPH HRHQSDKNPG SSNLQKITKE PIIDYFDVQD) of Human NFX1 (NP_002495).

  • 阳性对照
    • HepG2 cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab83833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 124 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. It may play a role in the inflammatory response, regulating its duration by limiting the period in which class II MHC molecules are induced by IFN gamma. The RING type zinc finger domain interacts with an ubiquitin conjugating enzyme (E2) and facilitates ubiquitination.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • MGC20369 antibody
    • NFX 1 antibody
    • NFX1 antibody
    • NFX2 antibody
    • Nuclear transcription factor X box binding 1 antibody
    • Nuclear transcription factor X box binding protein 1 antibody
    • Transcriptional repressor NF X1 antibody
    see all

Anti-NFX1 antibody 图像

  • Anti-NFX1 antibody (ab83833) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 124 kDa
    Observed band size : 124 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration 6-18%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling NFX1 with ab83833 at 5µg/ml.

Anti-NFX1 antibody (ab83833)参考文献

ab83833 has not yet been referenced specifically in any publications.

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