The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 151 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold.
组织特异性Expressed in heart, skeletal muscle and tracheal epithelial cells.
序列相似性Belongs to the Tonsoku family. Contains 3 ANK repeats. Contains 7 LRR (leucine-rich) repeats. Contains 8 TPR repeats.
结构域The ANK repeats mediate the interaction with the MCM complex and histones, while the LRR repeats mediate the interaction with MMS22L.
细胞定位Cytoplasm. Nucleus. Mainly nuclear. Localizes to DNA damage sites, accumulates at stressed replication forks.