Anti-NFkB p105 / p50抗体(ab31410)

概述

  • 产品名称Anti-NFkB p105 / p50抗体
    参阅全部 NFkB p105 / p50 一抗
  • 描述
    兔多克隆抗体to NFkB p105 / p50
  • 特异性Antibody detects endogenous levels of NFkB p105/p50 protein around Serine 337.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human NFkB p105/ p50. Synthetic non-phosphopeptide derived from Human NFkB p105/p50 around the phosphorylation site of Serine 337.
    Database link: P19838

  • 阳性对照

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab31410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/500. Predicted molecular weight: 105 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

靶标

  • 功能NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
  • 序列相似性Contains 7 ANK repeats.
    Contains 1 death domain.
    Contains 1 RHD (Rel-like) domain.
  • 结构域The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation.
    Glycine-rich region (GRR) appears to be a critical element in the generation of p50.
  • 翻译后修饰While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.
    Phosphorylation at 'Ser-903' and 'Ser-907' primes p105 for proteolytic processing in response to TNF-alpha stimulation. Phosphorylation at 'Ser-927' and 'Ser-932' are required for BTRC/BTRCP-mediated proteolysis.
    Polyubiquitination seems to allow p105 processing.
    S-nitrosylation of Cys-61 affects DNA binding.
  • 细胞定位Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor.
  • Information by UniProt
  • 数据库链接
  • 别名
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    • EBP 1 antibody
    • EBP-1 antibody
    • EBP1 antibody
    • KBF1 antibody
    • MGC54151 antibody
    • NF kappa B antibody
    • NF kappaB antibody
    • NF kappabeta antibody
    • NF kB1 antibody
    • NFkappaB antibody
    • NFKB 1 antibody
    • NFKB p105 antibody
    • NFKB p50 antibody
    • Nfkb1 antibody
    • NFKB1_HUMAN antibody
    • Nuclear factor kappa B DNA binding subunit antibody
    • Nuclear factor kappa-B, subunit 1 antibody
    • Nuclear factor NF kappa B p105 subunit antibody
    • Nuclear factor NF kappa B p50 subunit antibody
    • Nuclear factor NF-kappa-B p50 subunit antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells 1 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody
    • p105 antibody
    • p50 antibody
    • p84/NF-kappa-B1 p98 antibody
    • Transcription factor NFKB1 antibody
    see all

Anti-NFkB p105 / p50 antibody 图像

  • ab31410 at 1/50, staining NFkB p105/50 in human breast carcinoma using Immunohistochemistry, Paraffin Embedded Tissue. Left image : Un-treated. Right image : Antibody pre-incubated with synthesized peptide.
  • All lanes : Anti-NFkB p105 / p50 antibody (ab31410) at 1/200 dilution

    Lane 1 : Extracts of MDA-MB-435 cells.
    Lane 2 : Extracts of MDA-MB-435 cells. with pre-incubated with synthesized peptide.


    Predicted band size : 105 kDa

Anti-NFkB p105 / p50 antibody (ab31410)参考文献

ab31410 has not yet been referenced specifically in any publications.

Product Wall

Thank you for clarifying that.

I am also not an expert in IHC staining but I have consulted with my colleague who has extensive experience in this area. She has confirmed that while the staining may well be biologically significant, using dif...

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Thank you for contacting us.

Unfortunately I was not able to find any further correspondence between the customer and ourselves in regards to how the staining changed or if the customer tried the protocol provided. If there was any further co...

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Thank you for contacting us.

There are 3 antibodies we can offer ab31410, ab28849 and ab7971. The immunogen of these antibodies is significantly similar to Porcine protein http://www.uniprot.org/uniprot/Q0PHA8 so these antibodies might cross r...

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Thank you for you telephone enquiry. I am unable to provide the full amino acid sequence of the immunogen for this antibody (ab31410). However, I can confirm it is 16aa peptide containing R-K-S-D-L. This is a synthesized non-phosphopeptide derived ...

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We have an answer: Thank you for your response. I have some further information relating to the problems that you have experienced 1)This Ab is used to stain human breast cancer tissue often. There were three different results could obtained: nucl...

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Thank you for your response. I have some further information relating to the problems that you have experienced 1)This Ab is used to stain human breast cancer tissue often. There were three different results could obtained: nuclear staining, cytopl...

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