Anti-NFkB p100/NFKB2 (phospho S870)抗体(ab194924)
Key features and details
- Rabbit polyclonal to NFkB p100/NFKB2 (phospho S870)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-NFkB p100/NFKB2 (phospho S870)抗体
参阅全部 NFkB p100/NFKB2 一抗 -
描述
兔多克隆抗体to NFkB p100/NFKB2 (phospho S870) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human NFkB p100/NFKB2 (phospho S870). The exact sequence is proprietary.
Database link: Q00653 -
阳性对照
- WB: TNF-alpha treated MDA-MB-435 cell lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab194924于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 97 kDa.
|
说明 |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 97 kDa. |
靶标
-
相关性
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. -
细胞定位
Cytoplasmic and Nuclear -
数据库链接
- Entrez Gene: 4791 Human
- Omim: 164012 Human
- SwissProt: Q00653 Human
-
别名
- CVID10 antibody
- DNA binding factor KBF2 antibody
- H2TF1 antibody
see all
图片
-
All lanes : Anti-NFkB p100/NFKB2 (phospho S870) antibody (ab194924)
Lane 1 : Untreated MDA-MB-435 cell lysate
Lane 2 : TNF-alpha treated MDA-MB-435 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking buffer: 3% BSA.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab194924 尚未被引用在任何文献中。