Anti-NFkB Inducing Kinase NIK抗体(ab7204)


  • 产品名称Anti-NFkB Inducing Kinase NIK抗体
    参阅全部 NFkB Inducing Kinase NIK 一抗
  • 描述
    兔多克隆抗体to NFkB Inducing Kinase NIK
  • 经测试应用适用于: ICC/IF, IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human NFkB Inducing Kinase NIK aa 795-947.
    Database link: Q99558



Our Abpromise guarantee covers the use of ab7204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ICC/IF: Use at a concentration of 5 µg/ml.
    IP: 1/500.
    WB: 1/2000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
    • 组织特异性Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
    • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
      Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • MAP3K14 antibody
      • FTDCR1B antibody
      • HS antibody
      • HsNIK antibody
      • M3K14_HUMAN antibody
      • Map3k14 antibody
      • Mitogen activated protein kinase kinase kinase 14 antibody
      • Mitogen-activated protein kinase kinase kinase 14 antibody
      • NF kappa beta inducing kinase antibody
      • NF-kappa-beta-inducing kinase antibody
      • NIK antibody
      • Serine/threonine-protein kinase NIK antibody
      see all

    Anti-NFkB Inducing Kinase NIK antibody 图像

    • All lanes : Anti-NFkB Inducing Kinase NIK antibody (ab7204)

      Lane 1 : macrophages lysate
      Lane 2 : NIK transfected cell lysate
    • ICC/IF image of ab7204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-NFkB Inducing Kinase NIK antibody (ab7204)参考文献

    This product has been referenced in:
    • Döppler H  et al. Downregulation of TRAF2 mediates NIK-induced pancreatic cancer cell proliferation and tumorigenicity. PLoS One 8:e53676 (2013). WB, IHC-P ; Human . Read more (PubMed: 23301098) »
    • Ranuncolo SM  et al. Hodgkin lymphoma requires stabilized NIK and constitutive RelB expression for survival. Blood 120:3756-63 (2012). Read more (PubMed: 22968463) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Mouse Cell lysate - whole cell (colon)
    Specification colon
    Treatment overexpression
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

    Liwei Xie

    Verified customer

    提交于 Apr 23 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293)
    Loading amount 20 µg
    Specification HEK293
    Gel Running Conditions Non-reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Abcam user community

    Verified customer

    提交于 Jul 16 2012

    Thank you for your secondary. Ab7092 - Goat polyclonal to Rat IgG H&L Pre-Adsorbed - is a secondary antibody and is not a positive control for ab7204. Ab7204 was characterized for application in Western blotting using NIK transfected cell lysate wh...

    Read More

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. Should you decide to go ahead and purchase this product, please le...

    Read More

    We haven't tested endogenous NIK but one of our customers has got this to reliably work for detecting endogenous NIK.