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Our Abpromise guarantee covers the use of ab19144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||1/500. Detects a band of approximately 105 kDa (predicted molecular weight: 105.4 kDa).Can be blocked with NFkB Inducing Kinase NIK peptide (ab8385).|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"