概述

性能

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应用

Our Abpromise guarantee covers the use of ab87981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • 序列相似性Belongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCAAT box binding transcription factor antibody
    • CCAAT-box-binding transcription factor antibody
    • CTF antibody
    • NF-I/X antibody
    • NF1-X antibody
    • NF1A antibody
    • NF1X antibody
    • NFI X antibody
    • NFI-X antibody
    • NFI/X antibody
    • NFIX antibody
    • NFIX_HUMAN antibody
    • Nuclear factor 1 X type antibody
    • Nuclear factor 1 X-type antibody
    • Nuclear factor 1/X antibody
    • Nuclear factor I/X antibody
    • TGGCA binding protein antibody
    • TGGCA-binding protein antibody
    see all

Anti-NFIX antibody 图像

Anti-NFIX antibody (ab87981)参考文献

This product has been referenced in:
  • Ferenczy MW  et al. Clonal immortalized human glial cell lines support varying levels of JC virus infection due to differences in cellular gene expression. J Neuroimmune Pharmacol 8:1303-19 (2013). Read more (PubMed: 24052414) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (E17.5 brain)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 30 µg
Specification E17.5 brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Feb 29 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (whole embryo E16.5)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Specification whole embryo E16.5
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative Paraformaldehyde
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Abcam user community

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提交于 Jan 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"