Anti-NFIL3抗体(ab88526)
Key features and details
- Rabbit polyclonal to NFIL3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NFIL3抗体
参阅全部 NFIL3 一抗 -
描述
兔多克隆抗体to NFIL3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human NFIL3 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab103932) -
阳性对照
- This antibody gave a positive signal in the following Human whole cell lysates: HeLa; K562.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88526于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 mg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
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说明 |
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WB
Use a concentration of 1 mg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa). |
靶标
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功能
Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death. -
组织特异性
Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle. -
序列相似性
Belongs to the bZIP family. NFIL3 subfamily.
Contains 1 bZIP domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4783 Human
- Omim: 605327 Human
- SwissProt: Q16649 Human
- Unigene: 79334 Human
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别名
- E4 promoter binding protein 4 antibody
- E4 promoter-binding protein 4 antibody
- E4BP 4 antibody
see all
图片
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All lanes : Anti-NFIL3 antibody (ab88526) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam has observed other anti-NFIL3 antibodies react with a 55 kDa band. Abcam welcomes customer feedback.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab88526 尚未被引用在任何文献中。