概述

  • 产品名称Anti-NFIC抗体
    参阅全部 NFIC 一抗
  • 描述
    小鼠单克隆抗体to NFIC
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 314 - 424 of Human NFIC (NP_005588) with a proprietary tag

  • 阳性对照
    • HeLa cells nuclear extracts

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab89516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • 序列相似性Belongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110019L22Rik antibody
    • 1500041O16Rik antibody
    • AA589446 antibody
    • AI746521 antibody
    • CAAT box transcription factor antibody
    • CCAAT binding transcription factor antibody
    • CCAAT box binding transcription factor antibody
    • CCAAT-box-binding transcription factor antibody
    • CNFI C antibody
    • CTF antibody
    • CTF5 antibody
    • MGC137374 antibody
    • MGC20153 antibody
    • NF I antibody
    • NF I/C antibody
    • NF-I/C antibody
    • NF1 C antibody
    • NF1-C antibody
    • NF1C antibody
    • NFI antibody
    • NFI-C antibody
    • NFI/C antibody
    • NFIC antibody
    • NFIC_HUMAN antibody
    • Nuclear factor 1 antibody
    • Nuclear factor 1 C type antibody
    • Nuclear factor 1 C-type antibody
    • Nuclear factor 1/C antibody
    • Nuclear factor I/C (CCAAT binding transcription factor) antibody
    • Nuclear factor I/C antibody
    • TGGCA binding protein antibody
    • TGGCA-binding protein antibody
    • Transcription factor NFIC antibody
    see all

Anti-NFIC antibody 图像

  • Anti-NFIC antibody (ab89516) at 1/500 dilution + immunogen (110aa recombinant fragment with a tag of 26kDa) at 0.2 µg

    Predicted band size : 56 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-NFIC antibody (ab89516) at 1/500 dilution + HeLa nuclear extracts at 50 µg

    Predicted band size : 56 kDa

Anti-NFIC antibody (ab89516)参考文献

This product has been referenced in:
  • Li D  et al. Inhibition of AHR transcription by NF1C is affected by a single-nucleotide polymorphism, and is involved in suppression of human uterine endometrial cancer. Oncogene : (2012). Read more (PubMed: 23208493) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse pancreas)
Permeabilization Yes - Triton
Specification Mouse pancreas
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Fixative Formaldehyde
Username

Isidoro

Verified customer

提交于 Feb 29 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (Murine primary cells)
Gel Running Conditions Reduced Denaturing (Gel 12%)
Loading amount 20 µg
Specification Murine primary cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Isidoro

Verified customer

提交于 Feb 24 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"